ArticleJournal of the American Chemical Society2010
Monitoring the escape of DNA from a nanopore using an alternating current signal.
Article in Journal of the American Chemical Society, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 90 citations in OpenAlex.
- Crowding induced switching of polymer translocation by the amalgamation of entropy and osmotic pressure.iScience · 2024Article
- Review
- Nanopore Impedance Spectroscopy Reveals Electrical Properties of Single Nanoparticles for Detecting and Identifying Pathogenic Viruses.ACS omega · 2023Article
- Laser-based temperature control to study the roles of entropy and enthalpy in polymer-nanopore interactions.Science advances · 2021Article
- Colloquium: Ionic phenomena in nanoscale pores through 2D materials.Reviews of modern physics · 2019Article
- γ-Hemolysin Nanopore Is Sensitive to Guanine-to-Inosine Substitutions in Double-Stranded DNA at the Single-Molecule Level.Journal of the American Chemical Society · 2018Article
- Single-Molecule Titration in a Protein Nanoreactor Reveals the Protonation/Deprotonation Mechanism of a C:C Mismatch in DNA.Journal of the American Chemical Society · 2018Article
- A light-regulated host-guest-based nanochannel system inspired by channelrhodopsins protein.Nature communications · 2017Article
- Charge, Diffusion, and Current Fluctuations of Single-Stranded DNA Trapped in an MspA Nanopore.Biophysical journal · 2017Article
- Mechanism of α-synuclein translocation through a VDAC nanopore revealed by energy landscape modeling of escape time distributions.Nanoscale · 2017Article
- Kinetics of T3-DNA Ligase-Catalyzed Phosphodiester Bond Formation Measured Using the α-Hemolysin Nanopore.ACS nano · 2016Article
- Low-voltage paper isotachophoresis device for DNA focusing.Lab on a chip · 2015Article
- Thermal Motion of DNA in an MspA Pore.Biophysical journal · 2015Article
- Ion channel probes for scanning ion conductance microscopy.Langmuir : the ACS journal of surfaces and colloids · 2014Article
- Antigen detection via the rate of ion current rectification change of the antibody-modified glass nanopore membrane.Langmuir : the ACS journal of surfaces and colloids · 2014Article
- Temperature and electrolyte optimization of the α-hemolysin latch sensing zone for detection of base modification in double-stranded DNA.Biophysical journal · 2014Article
- Microsecond simulations of DNA and ion transport in nanopores with novel ion-ion and ion-nucleotides effective potentials.Journal of computational chemistry · 2014Article
- Creating a Single Sensing Zone within an Alpha-Hemolysin Pore Via Site Directed Mutagenesis.BioNanoScience · 2014Article
- Article
- Escape of DNA from a weakly biased thin nanopore: experimental evidence for a universal diffusive behavior.Physical review letters · 2013Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
We present the use of an alternating current (AC) signal as a means to monitor the conductance of an alpha-hemolysin (alphaHL) pore as a DNA hairpin with a polydeoxyadenosine tail is driven into and released from the pore. Specifically, a 12 base pair DNA hairpin attached to a 50-nucleotide poly-A tail (HP-A(50)) is threaded into an alphaHL channel using a DC driving voltage. Once the HP-A(50) molecule is trapped within the alphaHL channel, the DC driving voltage is turned off and the conductance of the channel is monitored using an AC voltage. The escape time, defined as the time it takes the HP-A(50) molecule to transport out of the alphaHL channel, is then measured. This escape time has been monitored as a function of AC amplitude (20 to 250 mV(ac)), AC frequency (60-200 kHz), DC drive voltage (0 to 100 mV(dc)), and temperature (-10 to 20 degrees C), in order to determine their effect on the predominantly diffusive motion of the DNA through the nanopore. The applied AC voltage used to monitor the conductance of the nanopore has been found to play a significant role in the DNA/nanopore interaction. The experimental results are described by a one-dimensional asymmetric periodic potential model that includes the influence of the AC voltage. An activation enthalpy barrier of 1.74 x 10(-19) J and a periodic potential asymmetry parameter of 0.575 are obtained for the diffusion at zero electrical bias of a single nucleotide through alphaHL.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.