ArticleBiochemistry2010
Structural insights into the prereaction state of pyruvate decarboxylase from Zymomonas mobilis .
Article in Biochemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 47 citations in OpenAlex.
- C2α-carbanion-protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2-hydroxyacyl-CoA lyase.FEBS open bio · 2026Article
- Engineering of 2-ketoacid Decarboxylases for Production of Isobutanol and Other Fusel Alcohols in Saccharomyces cerevisiae.Biotechnology and bioengineering · 2026Article
- Enzyme Architecture: Activation of Phosphite Dehydrogenase-Catalyzed Hydride Transfer by NADBiochemistry · 2025Article
- Improving tyrosol production efficiency through shortening the allosteric signal transmission distance of pyruvate decarboxylase.Applied microbiology and biotechnology · 2023Article
- Inhibition of Thiamine Diphosphate-Dependent Enzymes by Triazole-Based Thiamine Analogues.ACS medicinal chemistry letters · 2023Article
- New Role of Water in Transketolase Catalysis.International journal of molecular sciences · 2023Article
- Enzymes for Efficient COThe protein journal · 2021Review
- Crystal structure of an inferred ancestral bacterial pyruvate decarboxylase.Acta crystallographica. Section F, Structural biology communications · 2018Article
- An iterative computational design approach to increase the thermal endurance of a mesophilic enzyme.Biotechnology for biofuels · 2018Article
- Crystal structure of pyruvate decarboxylase from Zymobacter palmae.Acta crystallographica. Section F, Structural biology communications · 2016Article
- Redirection of the Reaction Specificity of a Thermophilic Acetolactate Synthase toward Acetaldehyde Formation.PloS one · 2016Article
- Structure and functional characterization of pyruvate decarboxylase from Gluconacetobacter diazotrophicus.BMC structural biology · 2014Article
- Observation of a stable carbene at the active site of a thiamin enzyme.Nature chemical biology · 2013Article
- Fluoride-mediated capture of a noncovalent bound state of a reversible covalent enzyme inhibitor: X-ray crystallographic analysis of an exceptionally potent α-ketoheterocycle inhibitor of fatty acid amide hydrolase.Journal of the American Chemical Society · 2011Article
- Experimental observation of thiamin diphosphate-bound intermediates on enzymes and mechanistic information derived from these observations.Bioorganic chemistry · 2005Review
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4 authors at 1 institution in 1 country.
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Abstract
Pyruvate decarboxylase (PDC) uses thiamine diphosphate as an essential cofactor to catalyze the formation of acetaldehyde on the pathway of ethanol synthesis. Here we report the crystallographic image of a prereaction intermediate of a bacterial pyruvate decarboxylase prepared by cocrystallizing the enzyme with pyruvate and a stable analogue of the cofactor's activated ylid form. A second crystal structure of PDC in complex with fluoride shows that the ion organizes a water molecule that occludes the pyruvate binding site, accounting for the inhibitory effect of the halide. Also reported is a structure of the cofactor-free apo form, which when compared to the structure of the holo form indicates how thiamine diphosphate organizes the active site pocket of pyruvate decarboxylase to support catalysis. Guided by the structural and enzymatic data, we propose roles for several key residues in the catalytic mechanism.
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