ArticleTransgenic research2010
Varying phenotypes in swine versus murine transgenic models constitutively expressing the same human Sonic hedgehog transcriptional activator, K5-HGLI2 Delta N.
Article in Transgenic research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- Genetically Engineered Pigs to Study Cancer.International journal of molecular sciences · 2020Review
- Generation of a TP53-modified porcine cancer model by CRISPR/Cas9-mediated gene modification in porcine zygotes via electroporation.PloS one · 2018Article
- Psoriasiform skin disease in transgenic pigs with high-copy ectopic expression of human integrins α2 and β1.Disease models & mechanisms · 2017Article
- Engineered Swine Models of Cancer.Frontiers in genetics · 2016Review
- Emerging Technologies to Create Inducible and Genetically Defined Porcine Cancer Models.Frontiers in genetics · 2016Review
- From reflux esophagitis to Barrett's esophagus and esophageal adenocarcinoma.World journal of gastroenterology · 2015Review
- The oncogenic GLI transcription factors facilitate keratinocyte survival and transformation upon exposure to genotoxic agents.Oncogene · 2014Article
- Genetically modified pigs to model human diseases.Journal of applied genetics · 2014Review
- From "ES-like" cells to induced pluripotent stem cells: a historical perspective in domestic animals.Theriogenology · 2014Review
- The new pig on the block: modelling cancer in pigs.Transgenic research · 2013Review
- Genetically engineered pig models for human diseases.Annual review of animal biosciences · 2013Review
- Genetically modified pigs for biomedical research.Journal of inherited metabolic disease · 2012Review
- Review: Experimental models for Barrett's esophagus and esophageal adenocarcinoma.American journal of physiology. Gastrointestinal and liver physiology · 2012Review
- Transgenic pigs as models for translational biomedical research.Journal of molecular medicine (Berlin, Germany) · 2010Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study was undertaken to characterize the effects of constitutive expression of the hedgehog transcriptional activator, Gli2, in porcine skin. The keratinocyte-specific human transgene, K5-hGli2 Delta N, was used to produce transgenic porcine lines via somatic cell nuclear transfer techniques. In mice, K5-hGli2 Delta N induces epithelial downgrowths resembling basal cell carcinomas. Our porcine model also developed these basal cell carcinoma-like lesions, however gross tumor development was not appreciated. In contrast to the murine model, diffuse epidermal changes as well as susceptibility to cutaneous infections were seen in the swine model. Histologic analysis of transgenic piglets revealed generalized epidermal changes including: epidermal hyperplasia (acanthosis), elongated rete ridges, parakeratotic hyperkeratosis, epidermal neutrophilic infiltration, capillary loop dilation and hypogranulosis. By 2 weeks of age, the transgenic piglets developed erythematic and edematous lesions at high contact epidermal areas and extensor surfaces of distal limb joints. Despite antibiotic treatment, these lesions progressed to a deep bacterial pyoderma and pigs died or were euthanized within weeks of birth. Non-transgenic littermates were phenotypically normal by gross and histological analysis. In summary, constitutive expression of the human hGli2 Delta N in keratinocytes, results in cutaneous changes that have not been reported in the K5-hGli2 Delta N murine model. These findings indicate a need for a multiple species animal model approach in order to better understand the role of Gli2 in mammalian skin.
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