ArticleEcotoxicology (London, England)2010
Introduction of foreign DNA into the water flea, Daphnia magna, by electroporation.
Article in Ecotoxicology (London, England), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed.
- Delivery methods for CRISPR/Cas9 gene editing in crustaceans.Marine life science & technology · 2020Article
- Protein Delivery into Plant Cells: TowardFrontiers in plant science · 2017Article
- Optimization of mRNA design for protein expression in the crustacean Daphnia magna.Molecular genetics and genomics : MGG · 2014Article
- Early embryonic expression of a putative ecdysteroid-phosphate phosphatase in the water flea, Daphnia magna (Cladocera: Daphniidae).Journal of insect science (Online) · 2014Article
- Daphnia as an emerging epigenetic model organism.Genetics research international · 2012Article
- The Epigenetic Repertoire of Daphnia magna Includes Modified Histones.Genetics research international · 2012Article
- Development of an RNA interference method in the cladoceran crustacean Daphnia magna.Development genes and evolution · 2011Article
- Environmental sex determination in the branchiopod crustacean Daphnia magna: deep conservation of a Doublesex gene in the sex-determining pathway.PLoS genetics · 2011Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Daphnids inhabit a diverse array of aquatic environments and they are a good model for understanding response and adaptation to environmental changes and they have been used one of standard organisms in ecotoxicology. Recent progress of genomics changed the tools for analyzing responses of daphnids, because gene expression changes can be observed before the emergence of prominent adverse effect such as immobility of the organism. Thus understanding of biological changes from gene expression level can be one of the sensitive tools for the evaluation of environmental response of organisms. However, there was no technique for genetic manipulation in daphnids. Hence, we have developed a gene introduction technique based on electroporation. There are two critical points for the successful introduction of foreign DNA into D. magna. (1) Injection of DNA into blood stream. (2) Usage of very low voltage for the electroporation. The injected DNA containing green fluorescent protein (GFP) could be introduced daphnids and the expression of GFP could be detected in living daphnids. This is the first report of gene introduction to daphnids and, together with the emerging genome sequences, will be useful for the expanding our use of daphnid in ecotoxicology.
Indexed as
Identifiers
20099028What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.