ArticlePloS one2010
Molecular interaction studies of trimethoxy flavone with human serum albumin.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 106 citations in OpenAlex.
- Roles of Virtual Screening and Molecular Dynamics Simulations in Discovering and Understanding Antimalarial Drugs.International journal of molecular sciences · 2023Review
- Food Antioxidants and Their Interaction with Human Proteins.Antioxidants (Basel, Switzerland) · 2023Review
- Spectroscopic Studies of Quinobenzothiazine Derivative in Terms of the In Vitro Interaction with Selected Human Plasma Proteins. Part 1.Molecules (Basel, Switzerland) · 2021Article
- Insights into the Binding of Dietary Phenolic Compounds to Human Serum Albumin and Food-Drug Interactions.Pharmaceutics · 2020Review
- Study of HSA interactions with arachidonic acid using spectroscopic methods revealing molecular dynamics of HSA-AA interactions.Biomedical reports · 2020Article
- Rapid Investigation and Screening of Bioactive Components in Simo Decoction via LC-Q-TOF-MS and UF-HPLC-MD Methods.Molecules (Basel, Switzerland) · 2018Article
- Screening and Identification for Immunological Active Components from Andrographis Herba Using Macrophage Biospecific Extraction Coupled with UPLC/Q-TOF-MS.Molecules (Basel, Switzerland) · 2018Article
- Elucidating the active interaction mechanism of phytochemicals withanolide and withanoside derivatives with human serum albumin.PloS one · 2018Article
- Effects of urea, metal ions and surfactants on the binding of baicalein with bovine serum albumin.Journal of pharmaceutical analysis · 2016Article
- Pre-infection administration of asiatic acid retards parasitaemia induction in Plasmodium berghei murine malaria infected Sprague-Dawley rats.Malaria journal · 2016Article
- Molecular structure-affinity relationship of bufadienolides and human serum albumin in vitro and molecular docking analysis.PloS one · 2015Article
- Interactive association of drugs binding to human serum albumin.International journal of molecular sciences · 2014Review
- Identification of Staphylococcus aureus colony-spreading stimulatory factors from mammalian serum.PloS one · 2014Article
- Molecular interaction mechanism between 2-mercaptobenzimidazole and copper-zinc superoxide dismutase.PloS one · 2014Article
- Preferential binding of fisetin to the native state of bovine serum albumin: spectroscopic and docking studies.Molecular biology reports · 2013Article
- Investigation of the interaction of naringin palmitate with bovine serum albumin: spectroscopic analysis and molecular docking.PloS one · 2013Article
- Elucidation of the binding mechanism of coumarin derivatives with human serum albumin.PloS one · 2013Article
- Molecular dynamics simulation studies of betulinic acid with human serum albumin.Journal of molecular modeling · 2012Article
- Effect of pullulan nanoparticle surface charges on HSA complexation and drug release behavior of HSA-bound nanoparticles.PloS one · 2012Article
- Binding of tetracycline and chlortetracycline to the enzyme trypsin: spectroscopic and molecular modeling investigations.PloS one · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHuman serum albumin (HSA) is the most abundant protein in blood plasma, having high affinity binding sites for several endogenous and exogenous compounds. Trimethoxy flavone (TMF) is a naturally occurring flavone isolated from Andrographis viscosula and used in the treatment of dyspepsia, influenza, malaria, respiratory functions and as an astringent and antidote for poisonous stings of some insects. METHODOLOGY/PRINCIPAL
findingsThe main aim of the experiment was to examine the interaction between TMF and HSA at physiological conditions. Upon addition of TMF to HSA, the fluorescence emission was quenched and the binding constant of TMF with HSA was found to be K(TMF) = 1.0+/-0.01x10(3) M(-1), which corresponds to -5.4 kcal M(-1) of free energy. Micro-TOF Q mass spectrometry results showed a mass increase of from 66,513 Da (free HSA) to 66,823 Da (HAS +Drug), indicating the strong binding of TMF with HSA resulting in decrease of fluorescence. The HSA conformation was altered upon binding of TMF to HSA with decrease in alpha-helix and an increase in beta-sheets and random coils suggesting partial unfolding of protein secondary structure. Molecular docking experiments found that TMF binds strongly with HSA at IIIA domain of hydrophobic pocket with hydrogen bond and hydrophobic interactions. Among which two hydrogen bonds are formed between O (19) of TMF to Arg 410, Tyr 411 and another one from O (7) of TMF to Asn 391, with bond distance of 2.1 A, 3.6 A and 2.6 A, respectively. CONCLUSIONS/SIGNIFICANCE: In view of the evidence presented, it is imperative to assign a greater role of HSA's as a carrier molecule for many drugs to understand the interactions of HSA with TMF will be pivotal in the design of new TMF-inspired drugs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.