ArticleThe Journal of biological chemistry2010
Dynamics of pre-replicative complex assembly.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 70 citations in OpenAlex.
- Unwinding Helicase MCM Functionality for Diagnosis and Therapeutics of Replication Abnormalities Associated with Cancer: A Review.Molecular diagnosis & therapy · 2024Review
- The SMC5/6 complex subunit MMS21 regulates stem cell proliferation in rice.Plant cell reports · 2023Article
- Regulation of replication origin licensing by ORC phosphorylation reveals a two-step mechanism for Mcm2-7 ring closing.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Regulation of replication origin licensing by ORC phosphorylation reveals a two-step mechanism for Mcm2-7 ring closing.bioRxiv : the preprint server for biology · 2023Article
- Exceptional origin activation revealed by comparative analysis in two laboratory yeast strains.PloS one · 2022Article
- Regulation of DNA Replication Licensing and Re-Replication by Cdt1.International journal of molecular sciences · 2021Review
- Unraveling CAF-1 family in3 Biotech · 2020Article
- Mapping the kinetochore MAP functions required for stabilizing microtubule attachments to chromosomes during metaphase.Cytoskeleton (Hoboken, N.J.) · 2019Review
- Direct binding of Cdt2 to PCNA is important for targeting the CRL4Life science alliance · 2018Article
- The Inherent Asymmetry of DNA Replication.Annual review of cell and developmental biology · 2017Review
- The High-Affinity Interaction between ORC and DNA that Is Required for Replication Licensing Is Inhibited by 2-Arylquinolin-4-Amines.Cell chemical biology · 2017Article
- Sap1 is a replication-initiation factor essential for the assembly of pre-replicative complex in the fission yeastThe Journal of biological chemistry · 2017Article
- Mechanisms and regulation of DNA replication initiation in eukaryotes.Critical reviews in biochemistry and molecular biology · 2017Review
- The Temporal Regulation of S Phase Proteins During GAdvances in experimental medicine and biology · 2017Review
- Replication-dependent and independent mechanisms for the chromosome-coupled persistence of a selfish genome.Nucleic acids research · 2016Article
- Diverged composition and regulation of the Trypanosoma brucei origin recognition complex that mediates DNA replication initiation.Nucleic acids research · 2016Article
- Simultaneous measurement of passage through the restriction point and MCM loading in single cells.Nucleic acids research · 2015Article
- Spatio-temporal regulation of the human licensing factor Cdc6 in replication and mitosis.Cell cycle (Georgetown, Tex.) · 2015Article
- Structural and mechanistic insights into Mcm2-7 double-hexamer assembly and function.Genes & development · 2014Article
- Multiple functions for Mcm2-7 ATPase motifs during replication initiation.Molecular cell · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The pre-replicative complex (pre-RC) is formed at all potential origins of replication through the action of the origin recognition complex (ORC), Cdc6, Cdt1, and the Mcm2-7 complex. The end result of pre-RC formation is the loading of the Mcm2-7 replicative helicase onto origin DNA. We examined pre-RC formation in vitro and found that it proceeds through separable binding events. Origin-bound ORC recruits Cdc6, and this ternary complex then promotes helicase loading in the presence of a pre-formed Mcm2-7-Cdt1 complex. Using a stepwise pre-RC assembly assay, we investigated the fate of pre-RC components during later stages of the reaction. We determined that helicase loading is accompanied by dissociation of ORC, Cdc6, and Cdt1 from origin DNA. This dissociation requires ATP hydrolysis at a late stage of pre-RC assembly. Our results indicate that pre-RC formation is a dynamic process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.