ArticleJournal of bacteriology2010
Pyruvate carboxylase plays a crucial role in carbon metabolism of extra- and intracellularly replicating Listeria monocytogenes.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
42 citing papers in PubMed, 77 citations in OpenAlex.
- Adaptive Tolerance of Listeria monocytogenes to Chemical Oxidants: Comparative Analysis of Transcriptomic Studies.Comprehensive reviews in food science and food safety · 2025Review
- Metabolic Master Switch: Pyruvate Carboxylase Fuels Antimicrobial Resistance and Virulence in FoodborneFoods (Basel, Switzerland) · 2025Article
- Exploring the Modulation of Extracellular Metabolites in DifferentFood science of animal resources · 2025Article
- Dysregulated biosynthesis and hydrolysis of cyclic-di-adenosine monophosphate impedes sporulation and butanol and acetone production inFrontiers in bioengineering and biotechnology · 2025Article
- C-di-AMP accumulation disrupts glutathione metabolism inInfection and immunity · 2024Article
- Oxaloacetate anaplerosis differently contributes to pathogenicity in plant pathogenic fungi Fusarium graminearum and F. oxysporum.PLoS pathogens · 2024Article
- Synthesis and Evaluation of 1,3-Disubstituted Imidazolidine-2,4,5-triones as Inhibitors of Pyruvate Carboxylase.ACS medicinal chemistry letters · 2024Article
- Bacterial cell volume regulation and the importance of cyclic di-AMP.Microbiology and molecular biology reviews : MMBR · 2024Review
- Allosteric Site at the Biotin Carboxylase Dimer Interface Mediates Activation and Inhibition inBiochemistry · 2023Article
- Enhanced Hemolytic Activity of MesophilicMicroorganisms · 2022Article
- The Biological Significance of Pyruvate Sensing and Uptake inMicroorganisms · 2022Article
- Functional Annotation of Hypothetical Proteins From theBioinformatics and biology insights · 2022Article
- Insights into a Pyruvate Sensing and Uptake System inJournal of bacteriology · 2021Article
- Fine Tuning of Phosphorothioate Inclusion in 2'-O-Methyl Oligonucleotides Contributes to Specific Cell Targeting for Splice-Switching Modulation.Frontiers in physiology · 2021Article
- Function and Regulation of the Pyruvate Transporter CstA inInternational journal of molecular sciences · 2020Article
- Targeting Pyruvate Carboxylase by a Small Molecule Suppresses Breast Cancer Progression.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Metabolism of the Gram-Positive Bacterial PathogenMicrobiology spectrum · 2019Review
- Article
- Review
- Roles of pyruvate carboxylase in human diseases: from diabetes to cancers and infection.Journal of molecular medicine (Berlin, Germany) · 2018Review
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The human pathogen L. monocytogenes is a facultatively intracellular bacterium that survives and replicates in the cytosol of many mammalian cells. The listerial metabolism, especially under intracellular conditions, is still poorly understood. Recent studies analyzed the carbon metabolism of L. monocytogenes by the (13)C isotopologue perturbation method in a defined minimal medium containing [U-(13)C(6)]glucose. It was shown that these bacteria produce oxaloacetate mainly by carboxylation of pyruvate due to an incomplete tricarboxylic acid cycle. Here, we report that a pycA insertion mutant defective in pyruvate carboxylase (PYC) still grows, albeit at a reduced rate, in brain heart infusion (BHI) medium but is unable to multiply in a defined minimal medium with glucose or glycerol as a carbon source. Aspartate and glutamate of the pycA mutant, in contrast to the wild-type strain, remain unlabeled when [U-(13)C(6)]glucose is added to BHI, indicating that the PYC-catalyzed carboxylation of pyruvate is the predominant reaction leading to oxaloacetate in L. monocytogenes. The pycA mutant is also unable to replicate in mammalian cells and exhibits high virulence attenuation in the mouse sepsis model.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.