ArticleApplied and environmental microbiology2010
Improved strains and plasmid vectors for conditional overexpression of His-tagged proteins in Haloferax volcanii.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 140 papers.
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Who cites it
140 citing papers in PubMed, 218 citations in OpenAlex.
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- Fluorescent probes as markers of cell envelope structure and function in halophilic archaea.Scientific reports · 2026Article
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- A Two-Component Regulatory System Mediates Quorum Sensing-Dependent Morphology and Motility Transitions in the ArchaeonbioRxiv : the preprint server for biology · 2025Article
- Biochemical properties of glycerol kinase from the hypersaline-adapted archaeonApplied and environmental microbiology · 2025Article
- Revisiting synthetic lethality of Gcn5-related N-acetyltransferase (GNAT) family mutations inMicrobiology spectrum · 2025Article
- Rapid rewiring of an archaeal transcription factor function via flexible cis-trans interactions.Molecular biology of the cell · 2025Article
- Review
- Molecular adaptations specific to extreme halophilic archaea could promote high perchlorate tolerance.Applied and environmental microbiology · 2025Article
- Cell division protein CdpA organises and anchors the midcell ring in haloarchaea.Nature communications · 2025Article
- Tissue-like multicellular development triggered by mechanical compression in archaea.Science (New York, N.Y.) · 2025Article
- Pharmaceutical applications of halophilic enzymes.Heliyon · 2025Review
- Tat-fimbriae ("tafi"): An unusual type of haloarchaeal surface structure depending on the twin-arginine translocation pathway.iScience · 2025Article
- Genomic re-sequencing reveals mutational divergence across genetically engineered strains of model archaea.mSystems · 2025Article
- CRISPR-Cas induced self-targeting identifies key players in archaeal microhomology-mediated end joining.microLife · 2025Review
- Rhomboid proteases: key players at the cell surface within haloarchaea.Frontiers in microbiology · 2025Article
80 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Research into archaea will not achieve its full potential until systems are in place to carry out genetics and biochemistry in the same species. Haloferax volcanii is widely regarded as the best-equipped organism for archaeal genetics, but the development of tools for the expression and purification of H. volcanii proteins has been neglected. We have developed a series of plasmid vectors and host strains for conditional overexpression of halophilic proteins in H. volcanii. The plasmids feature the tryptophan-inducible p.tnaA promoter and a 6xHis tag for protein purification by metal affinity chromatography. Purification is facilitated by host strains, where pitA is replaced by the ortholog from Natronomonas pharaonis. The latter lacks the histidine-rich linker region found in H. volcanii PitA and does not copurify with His-tagged recombinant proteins. We also deleted the mrr restriction endonuclease gene, thereby allowing direct transformation without the need to passage DNA through an Escherichia coli dam mutant.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.