ArticleApplied and environmental microbiology2010
Membrane topology of outer membrane protein AlgE, which is required for alginate production in Pseudomonas aeruginosa.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 37 citations in OpenAlex.
- Genome analysis of alginate synthesizing Pseudomonas aeruginosa strain SW1 isolated from degraded seaweeds.Antonie van Leeuwenhoek · 2021Article
- Identification of the Clostridial cellulose synthase and characterization of the cognate glycosyl hydrolase, CcsZ.PloS one · 2020Article
- Activation Mechanism and Cellular Localization of Membrane-Anchored Alginate Polymerase in Pseudomonas aeruginosa.Applied and environmental microbiology · 2017Article
- Discovery of Novel Leptospirosis Vaccine Candidates Using Reverse and Structural Vaccinology.Frontiers in immunology · 2017Article
- Biological function of a polysaccharide degrading enzyme in the periplasm.Scientific reports · 2016Article
- Alginate Biosynthesis Factories in Pseudomonas fluorescens: Localization and Correlation with Alginate Production Level.Applied and environmental microbiology · 2016Article
- Bactericidal Compounds Controlling Growth of the Plant Pathogen Pseudomonas syringae pv. actinidiae, Which Forms Biofilms Composed of a Novel Exopolysaccharide.Applied and environmental microbiology · 2015Article
- Bacterial exopolysaccharides: biosynthesis pathways and engineering strategies.Frontiers in microbiology · 2015Review
- Microbial alginate production, modification and its applications.Microbial biotechnology · 2013Review
- Role of PelF in pel polysaccharide biosynthesis in Pseudomonas aeruginosa.Applied and environmental microbiology · 2013Article
- Insights into the assembly of the alginate biosynthesis machinery in Pseudomonas aeruginosa.Applied and environmental microbiology · 2013Article
- Dual roles of Pseudomonas aeruginosa AlgE in secretion of the virulence factor alginate and formation of the secretion complex.Applied and environmental microbiology · 2013Article
- Article
- Mutational analyses reveal overall topology and functional regions of NilB, a bacterial outer membrane protein required for host association in a model of animal-microbe mutualism.Journal of bacteriology · 2012Article
- Structural basis for alginate secretion across the bacterial outer membrane.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Biosynthesis of the Pseudomonas aeruginosa Extracellular Polysaccharides, Alginate, Pel, and Psl.Frontiers in microbiology · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ubiquitous opportunistic human pathogen Pseudomonas aeruginosa secretes a viscous extracellular polysaccharide, called alginate, as a virulence factor during chronic infection of patients with cystic fibrosis. In the present study, it was demonstrated that the outer membrane protein AlgE is required for the production of alginate in P. aeruginosa. An isogenic marker-free algE deletion mutant was constructed. This strain was incapable of producing alginate but did secrete alginate degradation products, indicating that polymerization occurs but that the alginate chain is subsequently degraded during transit through the periplasm. Alginate production was restored by introducing the algE gene. The membrane topology of the outer membrane protein AlgE was assessed by site-specific insertions of FLAG epitopes into predicted extracellular loop regions.
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