ArticleCell calcium2010
Quantifying calcium fluxes underlying calcium puffs in Xenopus laevis oocytes.
Article in Cell calcium, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- Biological physics to uncover cell signaling.Biophysical reviews · 2025Review
- Luminal transport through intact endoplasmic reticulum limits the magnitude of localized CaProceedings of the National Academy of Sciences of the United States of America · 2024Article
- High rates of calcium-free diffusion in the cytosol of living cells.Biophysical journal · 2021Article
- Mode switching of Inositol 1,4,5-trisphosphate receptor channel shapes the Spatiotemporal scales of CaJournal of biological physics · 2016Article
- Modulation of elementary calcium release mediates a transition from puffs to waves in an IP3R cluster model.PLoS computational biology · 2015Article
- Article
- Basis for a neuronal version of Grover's quantum algorithm.Frontiers in molecular neuroscience · 2014Article
- A stochastic model of calcium puffs based on single-channel data.Biophysical journal · 2013Article
- Multi-scale data-driven modeling and observation of calcium puffs.Cell calcium · 2012Article
- How cells process information: quantification of spatiotemporal signaling dynamics.Protein science : a publication of the Protein Society · 2012Review
- Termination of Ca²+ release for clustered IP₃R channels.PLoS computational biology · 2012Article
- Signal mass and Ca²⁺ kinetics in local calcium events: a modeling study.Journal of molecular modeling · 2012Article
- Endogenous cytosolic Ca(2+) buffering is necessary for TRPM4 activity in cerebral artery smooth muscle cells.Cell calcium · 2012Article
- Nuclear membranes control symbiotic calcium signaling of legumes.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Inositol (1,4,5)-trisphosphate receptor microarchitecture shapes Ca2+ puff kinetics.Biophysical journal · 2011Article
- Mean field strategies induce unrealistic non-linearities in calcium puffs.Frontiers in physiology · 2011Article
- Unitary Ca(2+) current through recombinant type 3 InsP(3) receptor channels under physiological ionic conditions.The Journal of general physiology · 2010Article
- Intra-cluster percolation of calcium signals.PloS one · 2010Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
We determine the calcium fluxes through inositol 1,4,5-trisphosphate receptor/channels underlying calcium puffs of Xenopus laevis oocytes using a simplified version of the algorithm of Ventura et al. An analysis of 130 puffs obtained with Fluo-4 indicates that Ca2+ release comes from a region of width approximately 450 nm, that the release duration is peaked around 18 s and that the underlying Ca2+ currents range between 0.12 and 0.95 pA. All these parameters are independent of IP(3) concentration. We explore what distributions of channels that open during a puff, N(p), and what relations between current and number of open channels, I(N(p)), are compatible with our findings and with the distribution of puff-to-trigger amplitude ratio reported in Rose et al. To this end, we use simple "mean field" models in which all channels open and close simultaneously. We find that the variability among clusters plays an important role in shaping the observed puff amplitude distribution and that a model for which I(N(p)) approximately N(p) for small N(p) and I(N(p)) approximately N(p)(1/alpha) (alpha > 1) for large N(p), provides the best agreement. Simulations of more detailed models in which channels open and close stochastically show that this nonlinear behavior can be attributed to the limited time resolution of the observations and to the averaging procedure that is implicit in the mean-field models. These conclusions are also compatible with observations of approximately 400 puffs obtained using the dye Oregon green.
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