ArticleBiosensors & bioelectronics2010
Label-free microarray imaging for direct detection of DNA hybridization and single-nucleotide mismatches.
Article in Biosensors & bioelectronics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- Solid-Phase Optical Sensing Techniques for Sensitive Virus Detection.Sensors (Basel, Switzerland) · 2023Review
- Highly-Sensitive, Label-Free Detection of Microorganisms and Viruses via Interferometric Reflectance Imaging Sensor.Micromachines · 2023Review
- Article
- Critical assessment of relevant methods in the field of biosensors with direct optical detection based on fibers and waveguides using plasmonic, resonance, and interference effects.Analytical and bioanalytical chemistry · 2020Review
- Label-free tomography of living cellular nanoarchitecture using hyperspectral self-interference microscopy.Biomedical optics express · 2019Article
- Interferometric Reflectance Imaging Sensor (IRIS)--A Platform Technology for Multiplexed Diagnostics and Digital Detection.Sensors (Basel, Switzerland) · 2015Review
- Democratization of Nanoscale Imaging and Sensing Tools Using Photonics.Analytical chemistry · 2015Article
- Article
- TATA binding proteins can recognize nontraditional DNA sequences.Biophysical journal · 2012Article
- Interferometric methods for label-free molecular interaction studies.Analytical chemistry · 2012Review
- Synergetic chemiluminescence and label-free dual detection for developing a hepatitis protein array.Journal of immunological methods · 2011Article
- Biomolecular detection employing the Interferometric Reflectance Imaging Sensor (IRIS).Journal of visualized experiments : JoVE · 2011Article
- Label-free multiplexed virus detection using spectral reflectance imaging.Biosensors & bioelectronics · 2011Article
- Multiplexed, rapid point of care platform to quantify allergen-specific IgE.Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference · 2011Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
A novel method is proposed for direct detection of DNA hybridization on microarrays. Optical interferometry is used for label-free sensing of biomolecular accumulation on glass surfaces, enabling dynamic detection of interactions. Capabilities of the presented method are demonstrated by high-throughput sensing of solid-phase hybridization of oligonucleotides. Hybridization of surface immobilized probes with 20 base pair-long target oligonucleotides was detected by comparing the label-free microarray images taken before and after hybridization. Through dynamic data acquisition during denaturation by washing the sample with low ionic concentration buffer, melting of duplexes with a single-nucleotide mismatch was distinguished from perfectly matching duplexes with high confidence interval (>97%). The presented technique is simple, robust, and accurate, and eliminates the need of using labels or secondary reagents to monitor the oligonucleotide hybridization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.