ReviewMethods (San Diego, Calif.)2010
The design of Förster (fluorescence) resonance energy transfer (FRET)-based molecular sensors for Ran GTPase.
Review in Methods (San Diego, Calif.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 32 citations in OpenAlex.
- The Kinesin-14 tail: Dual microtubule binding domains drive spindle morphogenesis through tight microtubule cross-linking and robust sliding.Molecular biology of the cell · 2025Article
- Wearable Smart Contact Lenses for Continual Glucose Monitoring: A Review.Frontiers in medicine · 2022Review
- Using FLIM-FRET for Characterizing Spatial Interactions in the Spindle.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Article
- RanGTP induces an effector gradient of XCTK2 and importin α/β for spindle microtubule cross-linking.The Journal of cell biology · 2020Article
- In Vitro FRET- and Fluorescence-Based Assays to Study Protein Conformation and Protein-Protein Interactions in Mitosis.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Use of Laser Speckle Contrast Analysis during pelvic surgery in a uterine transplantation model.Future science OA · 2018Article
- Use of biomedical photonics in gynecological surgery: a uterine transplantation model.Future science OA · 2018Article
- Chromosomal gain promotes formation of a steep RanGTP gradient that drives mitosis in aneuploid cells.The Journal of cell biology · 2013Article
- Enhancement of probe signal for screening of HIV-1 protease inhibitors in living cells.Sensors (Basel, Switzerland) · 2012Article
- Overview of fluorescence glucose sensing: a technology with a bright future.Journal of diabetes science and technology · 2012Review
- How cells process information: quantification of spatiotemporal signaling dynamics.Protein science : a publication of the Protein Society · 2012Review
- Mitotic spindle assembly around RCC1-coated beads in Xenopus egg extracts.PLoS biology · 2011Article
- How kinesin-2 forms a stalk.Molecular biology of the cell · 2011Article
- Relative contributions of chromatin and kinetochores to mitotic spindle assembly.The Journal of cell biology · 2009Article
- FRAP, FLIM, and FRET: Detection and analysis of cellular dynamics on a molecular scale using fluorescence microscopy.Molecular reproduction and developmentReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
Abstract
The application of FRET-based molecular biosensors provided confirmation of the central model of Ran GTPase function and led to important new insights into its physiological role. In many fields of cell biology, methods employing FRET are a standard approach that is becoming increasingly accessible due to advances in instrumentation and available fluorophores. However, the optimal design of a FRET sensor remains to be the cornerstone of any successful FRET application. Utilizing the recent literature on FRET applications and our studies on Ran, we outline the basic considerations involved in designing molecular FRET sensors. We point to several broadly applicable principles that were used in many different FRET sensors that can detect a wide range of molecular events. Using the FRET sensors for Ran that we created as examples, we then focus on the practical aspects of FRET assays. We describe the preparation of a bipartite FRET sensor consisting of ECFP-Ran and EYFP-importin beta and its validation as a reporter for FRET-based high throughput screening in small molecule libraries. Finally, we review the design and optimization of monomolecular FRET sensors that monitor the RanGTP-RanBP1 interaction, and of sensors detecting the RanGTP-regulated importin beta cargo release.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.