ReviewMethods (San Diego, Calif.)2010
Direct physical study of kinetochore-microtubule interactions by reconstitution and interrogation with an optical force clamp.
Review in Methods (San Diego, Calif.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed.
- Kinetochore-microtubule attachments are strengthened by Cnn1 stabilization of Stu2.bioRxiv : the preprint server for biology · 2026Article
- Centromeres in the thermotolerant yeast K. marxianus mediate attachment to a single microtubule.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Article
- Force transmission through the inner kinetochore is enhanced by centromeric DNA sequences.eLife · 2025Article
- Article
- Mechanical coupling coordinates microtubule growth.bioRxiv : the preprint server for biology · 2023Article
- Article
- Three interacting regions of the Ndc80 and Dam1 complexes support microtubule tip-coupling under load.The Journal of cell biology · 2022Article
- Tension can directly suppress Aurora B kinase-triggered release of kinetochore-microtubule attachments.Nature communications · 2022Article
- Catching the Conformational Wave: Measuring the Working Strokes of Protofilaments as They Curl Outward from Disassembling Microtubule Tips.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Kinetochore-bound Mps1 regulates kinetochore-microtubule attachments via Ndc80 phosphorylation.The Journal of cell biology · 2021Article
- The microtubule-associated protein She1 coordinates directional spindle positioning by spatially restricting dynein activity.Journal of cell science · 2021Article
- Microtubule pivoting enables mitotic spindle assembly in S. cerevisiae.The Journal of cell biology · 2021Article
- The Proteomic Landscape of Centromeric Chromatin Reveals an Essential Role for the Ctf19Current biology : CB · 2021Article
- XMAP215 and γ-tubulin additively promote microtubule nucleation in purified solutions.Molecular biology of the cell · 2020Article
- Article
- Kinetochore-associated Stu2 promotes chromosome biorientation in vivo.PLoS genetics · 2019Article
- The kinetoplastid kinetochore protein KKT4 is an unconventional microtubule tip-coupling protein.The Journal of cell biology · 2018Article
- Direct measurement of the strength of microtubule attachment to yeast centrosomes.Molecular biology of the cell · 2017Article
- Article
- Low Efficiency Upconversion Nanoparticles for High-Resolution Coalignment of Near-Infrared and Visible Light Paths on a Light Microscope.ACS applied materials & interfaces · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
We detail our use of computer-controlled optical traps to study interactions between kinetochore components and dynamic microtubules. Over the last two decades optical traps have helped uncover the working principles of conventional molecular motors, such as kinesin and dynein, but only recently have they been applied to study kinetochore function. The most useful traps combine sensitive position detectors and servo-control, allowing them to be operated as force clamps that maintain constant loads on objects as they move. Our instrument, which is among the simplest designs that permits force clamping, relies on a computer-controlled piezoelectric stage and a single laser for trapping and position detection. We apply it in motility assays where beads coated with pure microtubule-binding kinetochore components are attached to the tips of individual dynamic microtubules. Like kinetochores in vivo, the beads remain tip-attached, undergoing movements coupled to filament assembly and disassembly. The force clamp provides many benefits over instruments that lack feedback control. It allows tension to be applied continuously during both assembly- and disassembly-driven movement, providing a close match to the physiological situation. It also enables tracking with high resolution, and simplifies data interpretation by eliminating artifacts due to molecular compliance. The formation of persistent, load-bearing attachments to dynamic microtubule tips is fundamental to all kinetochore activities. Our direct, physical study of kinetochore-microtubule coupling may therefore furnish insights into many vital kinetochore functions, including correction of aberrant attachments and generation of the 'wait-anaphase' signals that delay mitosis until all kinetochores are properly attached.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.