ArticleMicrobial pathogenesis
Early biofilm formation on microtiter plates is not correlated with the invasive disease potential of Streptococcus pneumoniae.
Article in Microbial pathogenesis. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Crotonylation driving Streptococcus pneumoniae adaption and virulence.Journal of advanced research · 2026Article
- Review
- High prevalence of antibiotic resistance and biofilm formation inIranian journal of microbiology · 2023Article
- A multiomics analysis of direct interkingdom dynamics between influenza A virus and Streptococcus pneumoniae uncovers host-independent changes to bacterial virulence fitness.PLoS pathogens · 2022Article
- Mechanisms Underlying Pneumococcal Transmission and Factors Influencing Host-Pneumococcus Interaction: A Review.Frontiers in cellular and infection microbiology · 2021Review
- Inhibition of Necroptosis to Prevent Long-term Cardiac Damage During Pneumococcal Pneumonia and Invasive Disease.The Journal of infectious diseases · 2020Article
- Influenza-Induced Oxidative Stress Sensitizes Lung Cells to Bacterial-Toxin-Mediated Necroptosis.Cell reports · 2020Article
- Necroptotic Cell Death Promotes Adaptive Immunity Against Colonizing Pneumococci.Frontiers in immunology · 2019Article
- Severity and properties of cardiac damage caused by Streptococcus pneumoniae are strain dependent.PloS one · 2018Article
- Increased Zinc Availability Enhances Initial Aggregation and Biofilm Formation ofFrontiers in cellular and infection microbiology · 2017Article
- Infiltrated Macrophages Die of Pneumolysin-Mediated Necroptosis following Pneumococcal Myocardial Invasion.Infection and immunity · 2016Article
- Dynamic capsule restructuring by the main pneumococcal autolysin LytA in response to the epithelium.Nature communications · 2016Article
- The Small Molecule DAM Inhibitor, Pyrimidinedione, Disrupts Streptococcus pneumoniae Biofilm Growth In Vitro.PloS one · 2015Article
- Streptococcus pneumoniae translocates into the myocardium and forms unique microlesions that disrupt cardiac function.PLoS pathogens · 2014Article
- Antibiotic activity against naive and induced Streptococcus pneumoniae biofilms in an in vitro pharmacodynamic model.Antimicrobial agents and chemotherapy · 2014Article
- Biofilm localization in the vertical wall of shaking 96-well plates.Scientifica · 2014Article
- Sinefungin, a natural nucleoside analogue of S-adenosylmethionine, inhibits Streptococcus pneumoniae biofilm growth.BioMed research international · 2014Article
- Pneumococci in biofilms are non-invasive: implications on nasopharyngeal colonization.Frontiers in cellular and infection microbiology · 2014Review
- Streptococcus pneumoniae biofilm formation and dispersion during colonization and disease.Frontiers in cellular and infection microbiology · 2014Review
- A live-attenuated pneumococcal vaccine elicits CD4+ T-cell dependent class switching and provides serotype independent protection against acute otitis media.EMBO molecular medicine · 2014Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Biofilm formation has been suggested to play an important role during Streptococcus pneumoniae nasopharyngeal colonization and may facilitate progression to pneumonia. To test whether the ability of S. pneumoniae to form biofilms was important for virulence we screened the ability of 30 invasive and 22 non-invasive clinical isolates of serotype 6A and 6B to form early biofilms on polystyrene microtiter plates and infect mice following intranasal and intratracheal challenge. We first determined that no correlation existed between the ability to form early biofilms and whether isolates were collected from healthy carriers or individuals with invasive disease. A disconnect between biofilm forming ability and the capacity to colonize the nasopharynx, cause pneumonia, and enter the bloodstream was also observed in mice. Importantly, S. pneumoniae mutants deficient in the established virulence determinants pneumolysin, CbpA, and hydrogen peroxide formed biofilms normally. Incidentally, we determined that robust biofilm production was dependent on the formation and coalescing of bacterial aggregates on a thin layer of bacteria attached to the plate surface. In summary, these studies suggest that the ability to form early biofilms in vitro does not reflect virulence potential. More complex studies are required to determine if biofilm formation is important for virulence.
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