ArticleExperimental gerontology2010
Defects in multiple complexes of the respiratory chain are present in ageing human colonic crypts.
Article in Experimental gerontology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 55 citations in OpenAlex.
- Variant load of mitochondrial DNA in single human mesenchymal stem cells.Scientific reports · 2024Article
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- Non-Genomic Hallmarks of Aging-The Review.International journal of molecular sciences · 2023Review
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- Hydrogen Sulfide Metabolizing Enzymes in the Intestinal Mucosa in Pediatric and Adult Inflammatory Bowel Disease.Antioxidants (Basel, Switzerland) · 2022Article
- Mitochondrial DNA mutations in ageing and cancer.Molecular oncology · 2022Review
- A subcellular cookie cutter for spatial genomics in human tissue.Analytical and bioanalytical chemistry · 2022Article
- Single-cell atlas of the aging mouse colon.iScience · 2022Article
- Automated quantitative high-throughput multiplex immunofluorescence pipeline to evaluate OXPHOS defects in formalin-fixed human prostate tissue.Scientific reports · 2022Article
- The Complicated Nature of Somatic mtDNA Mutations in Aging.Frontiers in aging · 2022Article
- Article
- Long-Term Caffeine Intake Exerts Protective Effects on Intestinal Aging by Regulating Vitellogenesis and Mitochondrial Function in an AgedNutrients · 2021Article
- Age-associated mitochondrial complex I deficiency is linked to increased stem cell proliferation rates in the mouse colon.Aging cell · 2021Article
- Heterologous Inferential Analysis (HIA) and Other Emerging Concepts: In Understanding Mitochondrial Variation In Pathogenesis: There is no More Low-Hanging Fruit.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Age-associated mitochondrial DNA mutations cause metabolic remodelling that contributes to accelerated intestinal tumorigenesis.Nature cancer · 2020Article
- Aberrant mitochondrial function in ageing and cancer.Biogerontology · 2020Review
- The rise and rise of mitochondrial DNA mutations.Open biology · 2020Review
- Age-Related Deterioration of Mitochondrial Function in the Intestine.Oxidative medicine and cellular longevity · 2020Review
- Effects of obesity and weight loss on mitochondrial structure and function and implications for colorectal cancer risk.The Proceedings of the Nutrition Society · 2019Article
- Inherited pathogenic mitochondrial DNA mutations and gastrointestinal stem cell populations.The Journal of pathology · 2018Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Mitochondrial DNA (mtDNA) mutations accumulate in a number of ageing tissues and are proposed to play a role in the ageing process. We have previously shown that colonic crypt stem cells accumulate somatic mtDNA point mutations during ageing. These mtDNA mutations result in the loss of the activity of complex IV (cytochrome c oxidase (COX)) of the respiratory chain in the stem cells and their progeny, producing colonic crypts which are entirely COX deficient. However it is not known whether the other complexes of the respiratory chain are similarly affected during ageing. Here we have used antibodies to individual subunits of complexes I-IV to investigate their expression in the colonic epithelium from human subjects aged 18-84. We show that in approximately 50% of crypts with any form of respiratory chain deficiency, decreased expression of subunits of multiple complexes is observed. Furthermore we have sequenced the entire mitochondrial genome of a number of cells with multiple complex defects and have found a wide variety of point mutations in these cells affecting a number of different protein encoding and RNA encoding genes. Finally we discuss the possible mechanisms by which multiple respiratory chain complex defects may occur in these cells.
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Registered trials
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