ArticleOtolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery2010
Hypertonic challenge to porcine vocal folds: effects on epithelial barrier function.
Article in Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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Who cites it
34 citing papers in PubMed, 55 citations in OpenAlex.
- Mouse Vocal Fold Permeability In Vivo: Effects of Novel Low-Tech Injury and Instillation Methods.The Laryngoscope · 2026Article
- Scoping review of the relationship between xerostomia and voice quality.European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2023Article
- Epithelial response to vocal fold microflap injury in a preclinical model.The Laryngoscope · 2023Article
- Hydrogel-Supported, Engineered Model of Vocal Fold Epithelium.ACS biomaterials science & engineering · 2021Article
- A Comparison of the Localization of Integral Membrane Proteins in Human and Rabbit Vocal Folds.The Laryngoscope · 2021Article
- Protein Substrate Alters Cell Physiology in Primary Culture of Vocal Fold Epithelial Cells.Cells, tissues, organs · 2021Article
- RNA sequencing identifies transcriptional changes in the rabbit larynx in response to low humidity challenge.BMC genomics · 2020Article
- Immunological tolerance of low-risk HPV in recurrent respiratory papillomatosis.Clinical and experimental immunology · 2020Review
- Unraveling the molecular pathobiology of vocal fold systemic dehydration using an in vivo rabbit model.PloS one · 2020Article
- Methodology for the establishment of primary porcine vocal fold epithelial cell cultures.The Laryngoscope · 2019Article
- Prolonged phonation impairs the integrity and barrier function of porcine vocal fold epithelium: a preliminary study.European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2018Article
- Magnetic resonance imaging quantification of dehydration and rehydration in vocal fold tissue layers.PloS one · 2018Article
- In vivo measurement of vocal fold surface resistance.The Laryngoscope · 2017Article
- An in vitro scaffold-free epithelial-fibroblast coculture model for the larynx.The Laryngoscope · 2017Article
- Recovery of Vocal Fold Epithelium after Acute Phonotrauma.Cells, tissues, organs · 2017Article
- In vivo investigation of acidified pepsin exposure to porcine vocal fold epithelia.The Laryngoscope · 2016Article
- Effects of phonation time and magnitude dose on vocal fold epithelial genes, barrier integrity, and function.The Laryngoscope · 2014Article
- Vocal fold epithelial barrier in health and injury: a research review.Journal of speech, language, and hearing research : JSLHR · 2014Review
- Systemic hydration: relating science to clinical practice in vocal health.Journal of voice : official journal of the Voice Foundation · 2014Review
- Vocal fold ion transport and mucin expression following acrolein exposure.The Journal of membrane biology · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
objectiveDehydration challenges can increase the chemical composition of surface fluid overlying vocal fold epithelia (hypertonic surface fluid). The vocal fold epithelium is posited to act as a barrier, shielding the lamina propria from perturbations in the airway lumen. However, the effects of hypertonic surface fluid on the barrier functions of vocal fold epithelia have not been quantified. We, therefore, sought to investigate whether hypertonic surface fluid compromises epithelial barrier function. We examined the effects of hypertonic surface fluid on vocal fold epithelial resistance, paracellular pathway morphology, and tight junction protein integrity. STUDY
designEx vivo, between group design.
settingLaboratory.
methodsPorcine vocal folds (n = 24) were exposed to hypertonic or isotonic challenge and examined by electrophysiology, transmission electron microscopy, and Western blot analyses.
resultsHypertonic, but not isotonic, challenge significantly reduced transepithelial resistance. This decrease in resistance was observed immediately after the challenge and was consistent with the appearance of dilated paracellular pathway morphology. However, hypertonic challenge did not alter protein levels of occludin, zona occludens-1, E-cadherin, or beta-catenin.
conclusionHypertonic surface fluid alters epithelial barrier function in the vocal folds. Specifically, exposure to hypertonic challenges increases epithelial permeability. Given the important role of the vocal fold epithelium in shielding the underlying mucosa from inhaled pathogens and pollutants, our data provide the impetus for future studies on pharmacological treatments aimed at restoring the hydration level and chemical composition of vocal fold surface fluid.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.