ArticleMicrobial cell factories2010
Isolation, characterization and heterologous expression of a novel chitosanase from Janthinobacterium sp. strain 4239.
Article in Microbial cell factories, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 68 citations in OpenAlex.
- Chitin degradation by the aquatic pigment-producing bacteria of Janthinobacterium spp. from stream flows and springs of Shulgan River basin and Shulgan-Tash Cave, Southern Urals.Biodegradation · 2025Article
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- Expression of chitosanase from Aspergillus fumigatus chitosanase in Saccharomyces cerevisiae by CRISPR-Cas9 tools.Bioresources and bioprocessing · 2024Article
- Crude Enzyme Concentrate of Filamentous Fungus Hydrolyzed Chitosan to Obtain Oligomers of Different Sizes.Polymers · 2023Article
- Gene cloning and molecular characterization of a thermostable chitosanase from Bacillus cereus TY24.BMC biotechnology · 2022Article
- Molecular characterization of a novel strain ofFrontiers in plant science · 2022Article
- Article
- Article
- Proteomic Analysis of Mycelial Exudates ofPathogens (Basel, Switzerland) · 2021Article
- Cloning and Characterization of a New Chitosanase From a Deep-Sea BacteriumFrontiers in microbiology · 2021Article
- Characterization of a New Chitosanase from a Marine Bacillus sp. and the Anti-Oxidant Activity of Its Hydrolysate.Marine drugs · 2020Article
- Cloning and Characterization of a Cold-adapted Chitosanase from Marine BacteriumMolecules (Basel, Switzerland) · 2019Article
- Purification and Characterization of A New Cold-Adapted and Thermo-Tolerant Chitosanase from Marine BacteriumMolecules (Basel, Switzerland) · 2019Article
- Diversity of family GH46 chitosanases in Kitasatospora setae KM-6054.Applied microbiology and biotechnology · 2017Article
- An Amphiprotic Novel Chitosanase from Bacillus mycoides and Its Application in the Production of Chitooligomers with Their Antioxidant and Anti-Inflammatory Evaluation.International journal of molecular sciences · 2016Article
- Fungal invasion of the rhizosphere microbiome.The ISME journal · 2016Article
- Discovery, Molecular Mechanisms, and Industrial Applications of Cold-Active Enzymes.Frontiers in microbiology · 2016Review
- Chitosanases from Family 46 of Glycoside Hydrolases: From Proteins to Phenotypes.Marine drugs · 2015Review
- Chitinolytic Bacteria-Assisted Conversion of Squid Pen and Its Effect on Dyes and Pigments Adsorption.Marine drugs · 2015Article
- Heterologous Expression and Efficient Secretion of Chitosanase from Microbacterium sp. in Escherichia coli.Indian journal of microbiology · 2015Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundChitosanases (EC 3.2.1.132) hydrolyze the polysaccharide chitosan, which is composed of partially acetylated beta-(1,4)-linked glucosamine residues. In nature, chitosanases are produced by a number of Gram-positive and Gram-negative bacteria, as well as by fungi, probably with the primary role of degrading chitosan from fungal and yeast cell walls for carbon metabolism. Chitosanases may also be utilized in eukaryotic cell manipulation for intracellular delivery of molecules formulated with chitosan as well as for transformation of filamentous fungi by temporal modification of the cell wall structures.However, the chitosanases used so far in transformation and transfection experiments show optimal activity at high temperature, which is incompatible with most transfection and transformation protocols. Thus, there is a need for chitosanases, which display activity at lower temperatures.
resultsThis paper describes the isolation of a chitosanase-producing, cold-active bacterium affiliated to the genus Janthinobacterium. The 876 bp chitosanase gene from the Janthinobacterium strain was isolated and characterized. The chitosanase was related to the Glycosyl Hydrolase family 46 chitosanases with Streptomyces chitosanase as the closest related (64% amino acid sequence identity). The chitosanase was expressed recombinantly as a periplasmic enzyme in Escherichia coli in amounts about 500 fold greater than in the native Janthinobacterium strain. Determination of temperature and pH optimum showed that the native and the recombinant chitosanase have maximal activity at pH 5-7 and at 45 degrees C, but with 30-70% of the maximum activity at 10 degrees C and 30 degrees C, respectively.
conclusionsA novel chitosanase enzyme and its corresponding gene was isolated from Janthinobacterium and produced recombinantly in E. coli as a periplasmic enzyme. The Janthinobacterium chitosanase displayed reasonable activity at 10 degrees C to 30 degrees C, temperatures that are preferred in transfection and transformation experiments.
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