ArticleJournal of microscopy2010
High-resolution wide-field microscopy with adaptive optics for spherical aberration correction and motionless focusing.
Article in Journal of microscopy, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Self-interference digital holography with computational aberration correction.Optics express · 2024Article
- Engineering photodynamics for treatment, priming and imaging.Nature reviews bioengineering · 2024Article
- 2.5D microscopy with polarization independent SLM for enhanced detection efficiency and aberration correction.Optics express · 2021Article
- 2.5D microscopy: Fast, high-throughput imaging via volumetric projection for quantitative subcellular analysis.ACS photonics · 2021Article
- Live feedback and 3D photoacoustic remote sensing.Quantitative imaging in medicine and surgery · 2021Article
- Addressing systematic errors in axial distance measurements in single-emitter localization microscopy.Optics express · 2020Article
- Aberration correction method based on double-helix point spread function.Journal of biomedical optics · 2018Article
- Super-Resolution Single Molecule FISH at the Drosophila Neuromuscular Junction.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Adaptive optics stochastic optical reconstruction microscopy (AO-STORM) by particle swarm optimization.Biomedical optics express · 2017Article
- High-NA open-top selective-plane illumination microscopy for biological imaging.Optics express · 2017Article
- Enhancing image quality in cleared tissue with adaptive optics.Journal of biomedical optics · 2016Article
- Characterisation of the effects of optical aberrations in single molecule techniques.Biomedical optics express · 2016Article
- High-resolution in-depth imaging of optically cleared thick samples using an adaptive SPIM.Scientific reports · 2015Article
- Article
- Extending single-molecule microscopy using optical Fourier processing.The journal of physical chemistry. B · 2014Article
- Wavefront sensorless adaptive optics temporal focusing-based multiphoton microscopy.Biomedical optics express · 2014Article
- Correction of depth-dependent aberrations in 3D single-molecule localization and super-resolution microscopy.Optics letters · 2014Article
- Guide-star-based computational adaptive optics for broadband interferometric tomography.Applied physics letters · 2012Article
- Computational adaptive optics for broadband optical interferometric tomography of biological tissue.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Three-dimensional imaging and photostimulation by remote-focusing and holographic light patterning.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Live imaging in cell biology requires three-dimensional data acquisition with the best resolution and signal-to-noise ratio possible. Depth aberrations are a major source of image degradation in three-dimensional microscopy, causing a significant loss of resolution and intensity deep into the sample. These aberrations occur because of the mismatch between the sample refractive index and the immersion medium index. We have built a wide-field fluorescence microscope that incorporates a large-throw deformable mirror to simultaneously focus and correct for depth aberration in three-dimensional imaging. Imaging fluorescent beads in water and glycerol with an oil immersion lens we demonstrate a corrected point spread function and a 2-fold improvement in signal intensity. We apply this new microscope to imaging biological samples, and show sharper images and improved deconvolution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.