ArticleActa oto-laryngologica2010
Adenovector-mediated hair cell regeneration is affected by promoter type.
Article in Acta oto-laryngologica, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Adenovector-mediated gene delivery to human umbilical cord mesenchymal stromal cells induces inner ear cell phenotype.Cellular reprogramming · 2013Trial
- Precise genetic control of ATOH1 enhances maturation of regenerated hair cells in the mature mouse utricle.Nature communications · 2024Article
- Improving Control of Gene Therapy-Based Neurotrophin Delivery for Inner Ear Applications.Frontiers in bioengineering and biotechnology · 2022Article
- Characterizing Adult Cochlear Supporting Cell Transcriptional Diversity Using Single-Cell RNA-Seq: Validation in the Adult Mouse and Translational Implications for the Adult Human Cochlea.Frontiers in molecular neuroscience · 2020Article
- Single Cell and Single Nucleus RNA-Seq Reveal Cellular Heterogeneity and Homeostatic Regulatory Networks in Adult Mouse Stria Vascularis.Frontiers in molecular neuroscience · 2019Article
- Organotypic Culture of Neonatal Murine Inner Ear Explants.Frontiers in cellular neuroscience · 2019Article
- Surgical method for virally mediated gene delivery to the mouse inner ear through the round window membrane.Journal of visualized experiments : JoVE · 2015Article
- Optimizing atoh1-induced vestibular hair cell regeneration.The Laryngoscope · 2014Article
- Lentivirus carrying the Atoh1 gene infects normal rat cochlea.Neural regeneration research · 2013Article
- Atoh1 induces auditory hair cell recovery in mice after ototoxic injury.The Laryngoscope · 2013Article
- Restoration of hearing in the VGLUT3 knockout mouse using virally mediated gene therapy.Neuron · 2012Article
- Atoh1, an essential transcription factor in neurogenesis and intestinal and inner ear development: function, regulation, and context dependency.Journal of the Association for Research in Otolaryngology : JARO · 2012Review
- Cochlear gene therapy.Current opinion in neurology · 2012Review
- Article
- A review of gene delivery and stem cell based therapies for regenerating inner ear hair cells.Journal of functional biomaterials · 2011Article
- Selective atonal gene delivery improves balance function in a mouse model of vestibular disease.Gene therapy · 2011Article
- Development of gene therapy for inner ear disease: Using bilateral vestibular hypofunction as a vehicle for translational research.Hearing research · 2011Review
- Adeno-associated virus-mediated gene delivery into the scala media of the normal and deafened adult mouse ear.Gene therapy · 2011Article
- Regeneration of Hair Cells: Making Sense of All the Noise.Pharmaceuticals (Basel, Switzerland) · 2011Article
- Conditional deletion of Atoh1 using Pax2-Cre results in viable mice without differentiated cochlear hair cells that have lost most of the organ of Corti.Hearing research · 2011Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
conclusionReplacement of vestibular hair cells induced by atoh1 driven by the tissue-specific GFAP promoter was significantly more efficient than use of the cBA or hCMV promoter.
objectiveTo test whether expression level, persistence, or selectivity from adenovirus vectors delivered in the inner ear can be altered by changing the adenovector backbone or by using different cellular and viral promoters. MATERIALS AND
methodsAdenovector and promoter modifications were tested for differences in transgene expression in adult macular organs. The effect of using an E1/E3 deleted vector was compared to E1/E3/E4 deleted vectors. The effect of using viral and cellular promoters to modify transgene expression was tested in explanted adult mouse macular organs. Based on these results three different promoters were tested for efficacy of atonal gene.
resultsUse of adenovectors containing human CMV, the hybrid cBA and ubiquitin promoters driving transgene expression resulted in different types of transgene expression. While several viral and cellular promoters provided broad cell type expression, expression driven by the GFAP promoter was limited to vestibular supporting cells, demonstrating the specificity of this promoter.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.