ArticleGenesis (New York, N.Y. : 2000)2010
Transgene excision in zebrafish using the phiC31 integrase.
Article in Genesis (New York, N.Y. : 2000), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
- There and turn back again: the application of phage serine integrases in eukaryotic systems.Frontiers in bioengineering and biotechnology · 2025Review
- Article
- Article
- Heterogeneity and genomic loci of ubiquitous transgenic Cre reporter lines in zebrafish.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Article
- Bi-FoRe: an efficient bidirectional knockin strategy to generate pairwise conditional alleles with fluorescent indicators.Protein & cell · 2021Article
- Protein trap: a new Swiss army knife for geneticists?Molecular biology reports · 2020Review
- ФC31 Integrase-Mediated Isolation and Characterization of Novel Safe Harbors for Transgene Expression in the Pig Genome.International journal of molecular sciences · 2018Article
- Multiple expression cassette exchange via TP901-1, R4, and Bxb1 integrase systems on a mouse artificial chromosome.FEBS open bio · 2017Article
- Review
- New applications for phage integrases.Journal of molecular biology · 2014Review
- Targeted transgene integration overcomes variability of position effects in zebrafish.Development (Cambridge, England) · 2014Article
- Efficient site-specific transgenesis and enhancer activity tests in medaka using PhiC31 integrase.Development (Cambridge, England) · 2013Article
- Highly parallel assays of tissue-specific enhancers in whole Drosophila embryos.Nature methods · 2013Article
- Site-directed zebrafish transgenesis into single landing sites with the phiC31 integrase system.Developmental dynamics : an official publication of the American Association of Anatomists · 2013Article
- Conditional gene manipulation: Cre-ating a new biological era.Journal of Zhejiang University. Science. B · 2012Review
- Streptomyces temperate bacteriophage integration systems for stable genetic engineering of actinomycetes (and other organisms).Journal of industrial microbiology & biotechnology · 2012Review
- Using ΦC31 integrase to mediate insertion of DNA in Xenopus embryos.Methods in molecular biology (Clifton, N.J.) · 2012Article
- ΦC31 integrase mediates efficient cassette exchange in the zebrafish germline.Developmental dynamics : an official publication of the American Association of Anatomists · 2011Article
- Transgenerational analysis of transcriptional silencing in zebrafish.Developmental biology · 2011Article
- Use of phage φC31 integrase as a tool for zebrafish genome manipulation.Methods in cell biology · 2011Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
Genome engineering strategies employing site-specific recombinases (SSRs) have become invaluable to the study of gene function in model organisms. One such SSR, the integrase encoded by the Streptomyces bacteriophage phiC31, promotes recombination between heterotypic attP and attB sites. In the present study I have examined the feasibility of the use of phiC31 integrase for intramolecular recombination strategies in zebrafish embryos. I report here that (1) phiC31 integrase is functional in zebrafish cells, (2) phiC31 integrase can excise a transgene cassette flanked by an attB and an attP site, analogous to a common use of the Cre/lox SSR system, (3) phiC31 integrase functions in the zebrafish germline, and (4) a phiC31 integrase-estrogen receptor hormone-binding domain variant fusion protein catalyzes attB-attP recombination in zebrafish embryos in a 4-hydroxytamoxifen-dependent manner, albeit less efficiently than phiC31 alone. These features should make this a useful approach for genome manipulations in the zebrafish.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.