ArticleApoptosis : an international journal on programmed cell death2010
Cytochrome c is rapidly reduced in the cytosol after mitochondrial outer membrane permeabilization.
Article in Apoptosis : an international journal on programmed cell death, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 55 citations in OpenAlex.
- Article
- Interruption of mitochondrial symbiosis is associated with the development of osteoporosis.Frontiers in endocrinology · 2025Review
- Phosphorylations and Acetylations of CytochromeCells · 2024Review
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- Window into the mind: Advanced handheld spectroscopic eye-safe technology for point-of-care neurodiagnostic.Science advances · 2023Article
- Article
- The Improvement of Functional State of Brain Mitochondria with Astaxanthin in Rats after Heart Failure.International journal of molecular sciences · 2022Article
- Article
- Double face of cytochrome c in cancers by Raman imaging.Scientific reports · 2022Article
- Decoding the role of cytochrome c in metabolism of human spermatozoa by Raman imaging.Frontiers in cell and developmental biology · 2022Article
- ROS networks: designs, aging, Parkinson's disease and precision therapies.NPJ systems biology and applications · 2020Article
- Effect of glycerol on photobleaching of cytochrome Raman lines in frozen yeast cells.European biophysics journal : EBJ · 2018Article
- Structural basis of mitochondrial dysfunction in response to cytochromeProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Therapeutic Potential of Novel Twin Compounds Containing Tetramethylpyrazine and Carnitine Substructures in Experimental Ischemic Stroke.Oxidative medicine and cellular longevity · 2017Article
- Redox State of Cytochromes in Frozen Yeast Cells Probed by Resonance Raman Spectroscopy.Biophysical journal · 2015Article
- MicroRNAs Regulate Mitochondrial Function in Cerebral Ischemia-Reperfusion Injury.International journal of molecular sciences · 2015Review
- ORF005L from infectious spleen and kidney necrosis virus is located in the inner mitochondrial membrane and induces apoptosis.Virus genes · 2014Article
- Regulation of the intrinsic apoptosis pathway by reactive oxygen species.Antioxidants & redox signaling · 2013Review
- Conformational change and human cytochrome c function: mutation of residue 41 modulates caspase activation and destabilizes Met-80 coordination.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2013Article
- The redox state of cytochrome c modulates resistance to methotrexate in human MCF7 breast cancer cells.PloS one · 2013Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Visible spectroscopy was used to measure real-time changes in the oxidation state of cytochrome c (cyt c) and the a-cytochromes (cyt aa(3)) of cytochrome oxidase during mitochondrial outer membrane permeabilization (MOMP) initiated by anisomycin in HL-60 cells. The oxidation state of mitochondrial cyt c was found to be approximately 62% oxidized before MOMP and became approximately 70% oxidized after MOMP. In contrast, the cytosolic pool of cyt c was found to be almost fully reduced. This oxidation change allows cyt c release to be continuously and quantitatively monitored in real time. Anoxia and antimycin were used to fully reduce and fully oxidize, respectively, the mitochondrial pool of cyt c and it was found that the release of cyt c was independent of it oxidation state consistent with a simple model of cyt c passively diffusing down a concentration gradient through a pore or tear in the outer membrane. After MOMP was complete, the flux of cyt c diffusing back into the mitochondria was measured from the residual mitochondrial oxygen consumption after complete inhibition of the bc(1) with antimycin and myxothiazol. The outer membrane was found to be highly permeable after MOMP implying that the reduction of cyt c in the cytosol must be very rapid. The permeability of the outer membrane measured in this study would result in the release of cyt c with a time constant of less than 1 s.
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