ArticleBiophysical journal1991
Growth conditions control the size and order of actin bundles in vitro.
Article in Biophysical journal, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Organization of a cytoskeletal superstructure in the apical domain of intestinal tuft cells.The Journal of cell biology · 2024Article
- Actin Bundles Dynamics and Architecture.Biomolecules · 2023Review
- Transgelin-2: A Double-Edged Sword in Immunity and Cancer Metastasis.Frontiers in cell and developmental biology · 2021Review
- TAGLN2 regulates T cell activation by stabilizing the actin cytoskeleton at the immunological synapse.The Journal of cell biology · 2015Article
- Correlation of actin crosslinker and capper expression levels with stereocilia growth phases.Molecular & cellular proteomics : MCP · 2014Article
- Thickness distribution of actin bundles in vitro.European biophysics journal : EBJ · 2008Article
- Intrinsic dynamic behavior of fascin in filopodia.Molecular biology of the cell · 2007Article
- How to make a curved Drosophila bristle using straight actin bundles.Proceedings of the National Academy of Sciences of the United States of America · 2005Review
- Stimulation of fascin spikes by thrombospondin-1 is mediated by the GTPases Rac and Cdc42.The Journal of cell biology · 2000Article
- Parallel actin bundles and their multiple actin-bundling proteins.Current opinion in cell biology · 2000Review
- F-actin bundles are derivatives of microvilli: What does this tell us about how bundles might form?The Journal of cell biology · 2000Review
- Espin contains an additional actin-binding site in its N terminus and is a major actin-bundling protein of the Sertoli cell-spermatid ectoplasmic specialization junctional plaque.Molecular biology of the cell · 1999Article
- Why are two different cross-linkers necessary for actin bundle formation in vivo and what does each cross-link contribute?The Journal of cell biology · 1998Article
- Small espin: a third actin-bundling protein and potential forked protein ortholog in brush border microvilli.The Journal of cell biology · 1998Article
- Drosophila singed, a fascin homolog, is required for actin bundle formation during oogenesis and bristle extension.The Journal of cell biology · 1994Article
- A 27,000-D core of the Dictyostelium 34,000-D protein retains Ca(2+)-regulated actin cross-linking but lacks bundling activity.The Journal of cell biology · 1993Article
- The actin filament severing protein actophorin promotes the formation of rigid bundles of actin filaments crosslinked with alpha-actinin.The Journal of cell biology · 1991Article
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2 authors.
Funding
Abstract
The bonding rules for actin filament bundles do not lead to a particular packing symmetry, but allow for either regular or disordered filament packing. Indeed, both hexagonal and disordered types of packing are observed in vivo. To investigate factors which control bundle order, as well as size, we examined the effect of protein concentration on the growth of actin-fascin bundles in vitro. We found that bundles require 4-8 d to achieve both maximum size and order. The largest and best ordered bundles were grown at low fascin and high actin concentrations (an initial fascin/actin ratio of 1:200). In contrast, a much larger number of poorly ordered bundles were formed at ratios of 1:25 and 1:50, and most surprisingly, no bundles were formed at 1:300 or 1:400. Based on these observations we propose a two-stage mechanism for bundle growth. The first stage is dominated by nucleation, which requires relatively high concentrations of fascin and which is therefore accompanied by rapid growth. Below some concentration threshold, nucleation ceases and bundles enter the second stage of slow growth, which continues until the supply of fascin is exhausted. By analogy with crystallization, we hypothesize that slower growth produces better order. We are able to use this mechanism to explain our observations as well as previous observations of bundle growth both in vitro and in vivo.
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