ArticleThe Journal of molecular diagnostics : JMD2010
A two-tube combined TaqMan/SYBR Green assay to identify mycobacteria and detect single global lineage-defining polymorphisms in Mycobacterium tuberculosis.
Article in The Journal of molecular diagnostics : JMD, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Genomic epidemiology of early SARS-CoV-2 transmission dynamics in Bangladesh.Virology journal · 2024Article
- Characterization of MicroRNA Expression Profiles and Identification of Potential Biomarkers in Leprosy.Journal of clinical microbiology · 2017Article
- Real time PCR for the rapid identification and drug susceptibility of Mycobacteria present in Bronchial washings.BMC infectious diseases · 2016Article
- Article
- Doubling Throughput of a Real-Time PCR.Scientific reports · 2015Article
- Oleoyl coenzyme A regulates interaction of transcriptional regulator RaaS (Rv1219c) with DNA in mycobacteria.The Journal of biological chemistry · 2014Article
- Face mask sampling for the detection of Mycobacterium tuberculosis in expelled aerosols.PloS one · 2014Article
- Altered serum microRNAs as biomarkers for the early diagnosis of pulmonary tuberculosis infection.BMC infectious diseases · 2012Article
- Resuscitation-promoting factors reveal an occult population of tubercle Bacilli in Sputum.American journal of respiratory and critical care medicine · 2010Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
We have developed a novel real-time PCR assay to identify and perform preliminary genotyping of mycobacteria in a manner tailored to our local service. Within a single thermocycler run, mycobacterial 16S rDNA and the Mycobacterium tuberculosis global lineage-defining RD750 polymorphism are targeted in separate reaction tubes, each of which includes both TaqMan and SYBR Green chemistries. The results of this 16S-RD assay differentiate M. tuberculosis complex (MTBC) from nontuberculous mycobacteria (NTM) and recognize whether or not MTBC isolates belong to the East African-Indian lineage, the single most frequently isolated global MTBC lineage in our service. If required, NTM amplicons may be sequenced to provide more specific identities. We report the technical performance of this assay on 88 mycobacteria-positive cultures and discuss its use in the initial management of mycobacterial infections. The 16S-RD assay correctly identified all 70 MTBC-positive cultures and 17 NTM-positive cultures while contemporaneously recognizing 26 MTBC isolates as within and 44 outside the East African-Indian lineage. In artificial samples, the combined assay also showed limited potential to detect mixed mycobacterial infections (MTBC/NTM) and tuberculosis infections involving more than one global MTBC lineage. The approach we have established can be readily tailored to targets of particular value for any mycobacterial diagnostic service, thereby optimizing the value of the results for local clinical and public health management of mycobacterial infections.
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Registered trials
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