Evidence map›Paper›PMID 20092637›Full record

ArticleVirology journal2010

A simple and rapid method for detection of Goose Parvovirus in the field by loop-mediated isothermal amplification.

JinLong Yang, Rui Yang, AnChun Cheng, MingShu Wang, LiZhi Fu, SongQuan Yang, SuHui Zhang, Liu Yang, ZhiYong Xu

Open access · goldAbstract readComparative StudyEvaluation Study
In one paragraph

Article in Virology journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
3.2field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 40 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 1 country.

JinLong YangChongqing Academy of Animal Science, Chongqing 402460, Chongqing, China.
Rui Yang
AnChun Cheng
MingShu Wang
LiZhi Fu
SongQuan Yang
SuHui Zhang
Liu Yang
ZhiYong Xu
Chongqing Academy of Animal Science · CNScience and Technology Department of Sichuan Province · CNHenan Institute of Science and Technology · CNSichuan Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundGoose parvovirus (GPV) is a Dependovirus associated with latent infection and mortality in geese. Currently, in a worldwide scale, GPV severely affects geese production. The objective of this study is to develop a loop-mediated isothermal amplification (LAMP) method for the sensitive, rapid, and inexpensive detection of GPV in the field.

resultsA set of six specific primers was designed by targeting the GPV VP3 DNA. With Bst DNA polymerase large fragment, the target DNA could be amplified at 65 degrees C as early as 20 min of incubation in a simple water bath. A positive reaction was identified through the detection of the LAMP product by color change visible to the naked eye. The detection limit of the assay was 28 copies/microl of plasmid pVP3, and with equal sensitivity and specificity to fluorescent quantitative real-time PCR (FQ-PCR).

conclusionsThe high sensitivity, specificity, and simplicity, as well as the high throughput, make this method suitable for specific detection of GPV infection in both field conditions and laboratory settings. The utilization of complicated equipment and conduct of technical training on the GPV LAMP were not necessary.

Indexed as

AnimalsBenzothiazolesDependovirusDiaminesDNA PrimersGeeseNucleic Acid Amplification TechniquesOrganic ChemicalsParvoviridae InfectionsPoultry DiseasesQuinolinesSensitivity and SpecificityStaining and LabelingTemperatureViral Structural ProteinsBenzothiazolesDiaminesDNA PrimersOrganic ChemicalsQuinolinesSYBR Green IViral Structural Proteins

Identifiers

PMID20092637
PMCPMC2829533
OpenAlexW1982612356

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.