ArticlePloS one2010
Identification of potential therapeutic targets for Burkholderia cenocepacia by comparative transcriptomics.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Glycoproteomic and proteomic analysis ofMicrobiology spectrum · 2024Article
- Transcriptional landscape ofMicrobial genomics · 2023Article
- The Burkholderia cenocepacia iron starvation σ factor, OrbS, possesses an on-board iron sensor.Nucleic acids research · 2022Article
- Burkholderia cenocepacia transcriptome during the early contacts with giant plasma membrane vesicles derived from live bronchial epithelial cells.Scientific reports · 2021Article
- Inter-replicon Gene Flow Contributes to Transcriptional Integration in theG3 (Bethesda, Md.) · 2018Article
- A c-di-GMP-Modulating Protein Regulates Swimming Motility ofFrontiers in cellular and infection microbiology · 2018Article
- The Divided Bacterial Genome: Structure, Function, and Evolution.Microbiology and molecular biology reviews : MMBR · 2017Review
- Review
- Iron Acquisition Mechanisms and Their Role in the Virulence ofFrontiers in cellular and infection microbiology · 2017Review
- Synthetic Cystic Fibrosis Sputum Medium Regulates Flagellar Biosynthesis through the flhF Gene in Burkholderia cenocepacia.Frontiers in cellular and infection microbiology · 2016Article
- Potential of metabolomics to reveal Burkholderia cepacia complex pathogenesis and antibiotic resistance.Frontiers in microbiology · 2015Review
- Analysis of Genome-scale Expression Network in Four Major Bacterial Residents of Cystic Fibrosis Lung.Current genomics · 2014Article
- Putrescine reduces antibiotic-induced oxidative stress as a mechanism of modulation of antibiotic resistance in Burkholderia cenocepacia.Antimicrobial agents and chemotherapy · 2014Article
- Comparative metabolic systems analysis of pathogenic Burkholderia.Journal of bacteriology · 2014Article
- Exploring the metabolic network of the epidemic pathogen Burkholderia cenocepacia J2315 via genome-scale reconstruction.BMC systems biology · 2011Article
- Defining a structural and kinetic rationale for paralogous copies of phenylacetate-CoA ligases from the cystic fibrosis pathogen Burkholderia cenocepacia J2315.The Journal of biological chemistry · 2011Article
- Burkholderia cenocepacia differential gene expression during host-pathogen interactions and adaptation to the host environment.Frontiers in cellular and infection microbiology · 2011Article
- The Burkholderia cenocepacia LysR-type transcriptional regulator ShvR influences expression of quorum-sensing, protease, type II secretion, and afc genes.Journal of bacteriology · 2011Article
- The type 2 secretion Pseudopilin, gspJ, is required for multihost pathogenicity of Burkholderia cenocepacia AU1054.Infection and immunity · 2010Article
- A decade of Burkholderia cenocepacia virulence determinant research.Infection and immunity · 2010Review
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Authors and funding
3 authors.
Funding
Abstract
backgroundBurkholderia cenocepacia is an endemic soil dweller and emerging opportunistic pathogen in patients with cystic fibrosis (CF). The identification of virulence factors and potential therapeutic targets has been hampered by the genomic diversity within the species as many factors are not shared among the pathogenic members of the species. METHODOLOGY/PRINCIPAL
findingsIn this study, global identification of putative virulence factors was performed by analyzing the transcriptome of two related strains of B. cenocepacia (one clinical, one environmental) under conditions mimicking cystic fibrosis sputum versus soil. Soil is a natural reservoir for this species; hence, genes induced under CF conditions relative to soil may represent adaptations that have occurred in clinical strains. Under CF conditions, several genes encoding proteins thought to be involved in virulence were induced and many new ones were identified. Our analysis, in combination with previous studies, reveals 458 strain-specific genes, 126 clinical-isolate-specific, and at least four species-specific genes that are induced under CF conditions. The chromosomal distribution of the induced genes was disproportionate to the size of the chromosome as genes expressed under soil conditions by both strains were more frequent on the second chromosome and those differentially regulated between strains were more frequent on the third chromosome. Conservation of these induced genes was established using the 11 available Bcc genome sequences to indicate whether potential therapeutic targets would be species-wide. CONCLUSIONS/SIGNIFICANCE: Comparative transcriptomics is a useful way to identify new potential virulence factors and therapeutic targets for pathogenic bacteria. We identified eight genes induced under CF conditions that were also conserved in the Bcc and may constitute particularly attractive therapeutic targets due to their signal sequence, predicted cellular location, and homology to known therapeutic targets.
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