ArticlePLoS pathogens2010
Within-host evolution of Burkholderia pseudomallei in four cases of acute melioidosis.
Article in PLoS pathogens, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed, 57 citations in OpenAlex.
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- Case Report: Genetic evolution ofWellcome open research · 2025Article
- Phenotypic and genetic alterations ofFrontiers in microbiology · 2023Article
- Comparison of Gut Microbiota Diversity Between Captive and Wild Tokay Gecko (Frontiers in microbiology · 2022Article
- A Persisting Nontropical Focus of Burkholderia pseudomallei with Limited Genome Evolution over Five Decades.mSystems · 2020Article
- In vitro passage alters virulence, immune activation and proteomic profiles of Burkholderia pseudomallei.Scientific reports · 2020Article
- Melioidosis fatalities in captive slender-tailed meerkats (Suricata suricatta): combining epidemiology, pathology and whole-genome sequencing supports variable mechanisms of transmission with one health implications.BMC veterinary research · 2019Article
- Comprehensive analysis of clinical Burkholderia pseudomallei isolates demonstrates conservation of unique lipid A structure and TLR4-dependent innate immune activation.PLoS neglected tropical diseases · 2018Article
- Melioidosis.Nature reviews. Disease primers · 2018Review
- Identification of sRNA mediated responses to nutrient depletion in Burkholderia pseudomallei.Scientific reports · 2017Article
- Whole-genome sequencing to investigate a non-clonal melioidosis cluster on a remote Australian island.Microbial genomics · 2017Article
- Differences in Inflammation Patterns Induced by African and AsianThe American journal of tropical medicine and hygiene · 2017Article
- Characterization of in vitro phenotypes of Burkholderia pseudomallei and Burkholderia mallei strains potentially associated with persistent infection in mice.Archives of microbiology · 2017Article
- Burkholderia pseudomallei: Challenges for the Clinical Microbiology Laboratory.Journal of clinical microbiology · 2016Review
- Whole-genome sequencing of a quarter-century melioidosis outbreak in temperate Australia uncovers a region of low-prevalence endemicity.Microbial genomics · 2016Article
- Genome Sequence of the Historical Clinical Isolate Burkholderia pseudomallei PHLS 6.Genome announcements · 2016Article
- Tracing melioidosis back to the source: using whole-genome sequencing to investigate an outbreak originating from a contaminated domestic water supply.Journal of clinical microbiology · 2015Article
- Characterization of Burkholderia pseudomallei Strains Using a Murine Intraperitoneal Infection Model and In Vitro Macrophage Assays.PloS one · 2015Article
- Microevolution of Burkholderia pseudomallei during an acute infection.Journal of clinical microbiology · 2014Article
- Analysis of Salmonella enterica serovar Typhimurium variable-number tandem-repeat data for public health investigation based on measured mutation rates and whole-genome sequence comparisons.Journal of bacteriology · 2014Article
Corrections and comments
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Authors and funding
16 authors at 3 institutions in 3 countries.
Funding
Abstract
Little is currently known about bacterial pathogen evolution and adaptation within the host during acute infection. Previous studies of Burkholderia pseudomallei, the etiologic agent of melioidosis, have shown that this opportunistic pathogen mutates rapidly both in vitro and in vivo at tandemly repeated loci, making this organism a relevant model for studying short-term evolution. In the current study, B. pseudomallei isolates cultured from multiple body sites from four Thai patients with disseminated melioidosis were subjected to fine-scale genotyping using multilocus variable-number tandem repeat analysis (MLVA). In order to understand and model the in vivo variable-number tandem repeat (VNTR) mutational process, we characterized the patterns and rates of mutations in vitro through parallel serial passage experiments of B. pseudomallei. Despite the short period of infection, substantial divergence from the putative founder genotype was observed in all four melioidosis cases. This study presents a paradigm for examining bacterial evolution over the short timescale of an acute infection. Further studies are required to determine whether the mutational process leads to phenotypic alterations that impact upon bacterial fitness in vivo. Our findings have important implications for future sampling strategies, since colonies in a single clinical sample may be genetically heterogeneous, and organisms in a culture taken late in the infective process may have undergone considerable genetic change compared with the founder inoculum.
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