ArticleMolecular biology of the cell2010
Gq-coupled purinergic receptors inhibit insulin-like growth factor-I/phosphoinositide 3-kinase pathway-dependent keratinocyte migration.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- Gαq Is the Specific Mediator of PAR-1 Transactivation of Kinase Receptors in Vascular Smooth Muscle Cells.International journal of molecular sciences · 2022Article
- The dual roles of autophagy and the GPCRs-mediating autophagy signaling pathway after cerebral ischemic stroke.Molecular brain · 2022Review
- Article
- Epidermal loss of Gαq confers a migratory and differentiation defect in keratinocytes.PloS one · 2017Article
- Human and mouse monocytes display distinct signalling and cytokine profiles upon stimulation with FFAR2/FFAR3 short-chain fatty acid receptor agonists.Scientific reports · 2016Article
- Dendritic epidermal T cells facilitate wound healing in diabetic mice.American journal of translational research · 2016Article
- G protein-coupled receptors and the regulation of autophagy.Trends in endocrinology and metabolism: TEM · 2014Review
- P2Y4 receptor-mediated pinocytosis contributes to amyloid beta-induced self-uptake by microglia.Molecular and cellular biology · 2013Article
- Differential roles of CXCL2 and CXCL3 and their receptors in regulating normal and asthmatic airway smooth muscle cell migration.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- PKD1 mediates negative feedback of PI3K/Akt activation in response to G protein-coupled receptors.PloS one · 2013Article
- Ecto-nucleoside triphosphate diphosphohydrolase 2 modulates local ATP-induced calcium signaling in human HaCaT keratinocytes.PloS one · 2013Article
- A comparison of epithelial-to-mesenchymal transition and re-epithelialization.Seminars in cancer biology · 2012Review
- New insights regarding the regulation of chemotaxis by nucleotides, adenosine, and their receptors.Purinergic signalling · 2012Review
- Nucleotide receptors as targets in the pharmacological enhancement of dermal wound healing.Purinergic signalling · 2011Article
- G alpha(q) signal in osteoblasts is inhibitory to the osteoanabolic action of parathyroid hormone.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Insulin-like growth factor-I (IGF-I) activation of phosphoinositol 3-kinase (PI3K) is an essential pathway for keratinocyte migration that is required for epidermis wound healing. We have previously reported that activation of Galpha((q/11))-coupled-P2Y(2) purinergic receptors by extracellular nucleotides delays keratinocyte wound closure. Here, we report that activation of P2Y(2) receptors by extracellular UTP inhibits the IGF-I-induced p110alpha-PI3K activation. Using siRNA and pharmacological inhibitors, we demonstrate that the UTP antagonistic effects on PI3K pathway are mediated by Galpha((q/11))-and not G((i/o))-independently of phospholipase Cbeta. Purinergic signaling does not affect the formation of the IGF-I receptor/insulin receptor substrate-I/p85 complex, but blocks the activity of a membrane-targeted active p110alpha mutant, indicating that UTP acts downstream of PI3K membrane recruitment. UTP was also found to efficiently attenuate, within few minutes, the IGF-I-induced PI3K-controlled translocation of the actin-nucleating protein cortactin to the plasma membrane. This supports the UTP ability to alter later migratory events. Indeed, UTP inhibits keratinocyte spreading and migration promoted by either IGF-I or a membrane-targeted active p110alpha mutant, in a Galpha(q/11)-dependent manner both. These findings provide new insight into the signaling cross-talk between receptor tyrosine kinase and Galpha((q/11))-coupled receptors, which mediate opposite effects on p110alpha-PI3K activity and keratinocyte migration.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.