ArticleMolecular biology of the cell2010
A specific form of phospho protein phosphatase 2 regulates anaphase-promoting complex/cyclosome association with spindle poles.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 41 citations in OpenAlex.
- DUSP12 promotes cell cycle progression and protects cells from ZNF622 mediated apoptosis.Cell death & disease · 2026Article
- Decoding protein phosphorylation during oocyte meiotic divisions using phosphoproteomics.eLife · 2025Article
- Human REXO4 is Required for Cell Cycle Progression.bioRxiv : the preprint server for biology · 2025Article
- The myosin regulatory light chain Myl5 localizes to mitotic spindle poles and is required for proper cell division.Cytoskeleton (Hoboken, N.J.) · 2021Article
- Phospho-regulation of mitotic spindle assembly.Cytoskeleton (Hoboken, N.J.) · 2020Review
- Phase Separation in Cell Division.Molecular cell · 2020Review
- Spindle Architectural Features Must Be Considered Along With Cell Size to Explain the Timing of Mitotic Checkpoint Silencing.Frontiers in physiology · 2020Article
- Menin Associates With the Mitotic Spindle and Is Important for Cell Division.Endocrinology · 2019Article
- Dissecting the mechanisms of cell division.The Journal of biological chemistry · 2019Review
- Leukemia Cell Cycle Chemical Profiling Identifies the G2-Phase Leukemia Specific Inhibitor Leusin-1.ACS chemical biology · 2019Article
- Mechanisms for the temporal regulation of substrate ubiquitination by the anaphase-promoting complex/cyclosome.Cell division · 2019Review
- A molecular cascade modulates MAP1B and confers resistance to mTOR inhibition in human glioblastoma.Neuro-oncology · 2018Article
- Microtubins: a novel class of small synthetic microtubule targeting drugs that inhibit cancer cell proliferation.Oncotarget · 2017Article
- Computational Cell Cycle Profiling of Cancer Cells for Prioritizing FDA-Approved Drugs with Repurposing Potential.Scientific reports · 2017Article
- "Wait anaphase" signals are not confined to the mitotic spindle.Molecular biology of the cell · 2017Article
- Fatostatin Inhibits Cancer Cell Proliferation by Affecting Mitotic Microtubule Spindle Assembly and Cell Division.The Journal of biological chemistry · 2016Article
- The X-Linked-Intellectual-Disability-Associated Ubiquitin Ligase Mid2 Interacts with Astrin and Regulates Astrin Levels to Promote Cell Division.Cell reports · 2016Article
- Global Phosphoproteomic Mapping of Early Mitotic Exit in Human Cells Identifies Novel Substrate Dephosphorylation Motifs.Molecular & cellular proteomics : MCP · 2015Article
- Spatiotemporal regulation of the anaphase-promoting complex in mitosis.Nature reviews. Molecular cell biology · 2015Review
- A unique insertion in STARD9's motor domain regulates its stability.Molecular biology of the cell · 2015Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In early mitosis, the END (Emi1/NuMA/Dynein-dynactin) network anchors the anaphase-promoting complex/cyclosome (APC/C) to the mitotic spindle and poles. Spindle anchoring restricts APC/C activity, thereby limiting the destruction of spindle-associated cyclin B and ensuring maintenance of spindle integrity. Emi1 binds directly to hypophosphorylated APC/C, linking the APC/C to the spindle via NuMA. However, whether the phosphorylation state of the APC/C is important for its association with the spindle and what kinases and phosphatases are necessary for regulating this event remain unknown. Here, we describe the regulation of APC/C-mitotic spindle pole association by phosphorylation. We find that only hypophosphorylated APC/C associates with microtubule asters, suggesting that phosphatases are important. Indeed, a specific form of PPP2 (CA/R1A/R2B) binds APC/C, and PPP2 activity is necessary for Cdc27 dephosphorylation. Screening by RNA interference, we find that inactivation of CA, R1A, or R2B leads to delocalization of APC/C from spindle poles, early mitotic spindle defects, a failure to congress chromosomes, and decreased levels of cyclin B on the spindle. Consistently, inhibition of cyclin B/Cdk1 activity increased APC/C binding to microtubules. Thus, cyclin B/Cdk1 and PPP2 regulate the dynamic association of APC/C with spindle poles in early mitosis, a step necessary for proper spindle formation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.