ArticleMolecular biology of the cell2010
Emi2 inhibition of the anaphase-promoting complex/cyclosome absolutely requires Emi2 binding via the C-terminal RL tail.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 41 citations in OpenAlex.
- MOS is a key regulator of meiotic midbody assembly and prevents abnormal divisions in mouse eggs and their polar bodies.Journal of cell science · 2026Article
- The oocyte zinc transportereLife · 2025Article
- The C-terminal domain of Emi2 conjugated to cell-penetrating peptide activates mouse oocyte.Frontiers in cell and developmental biology · 2025Article
- Zinc transporter ZnT3/Slc30a3 has a potential role in zinc ion influx in mouse oocytes.The Journal of reproduction and development · 2024Article
- Role of zinc in female reproduction.Biology of reproduction · 2021Review
- Calcineurin promotes APC/C activation at meiotic exit by acting on both XErp1 and Cdc20.EMBO reports · 2018Article
- The BTG4 and CAF1 complex prevents the spontaneous activation of eggs by deadenylating maternal mRNAs.Open biology · 2016Article
- TDM1 Regulation Determines the Number of Meiotic Divisions.PLoS genetics · 2016Article
- Spatiotemporal regulation of the anaphase-promoting complex in mitosis.Nature reviews. Molecular cell biology · 2015Review
- Functional characterization of Anaphase Promoting Complex/Cyclosome (APC/C) E3 ubiquitin ligases in tumorigenesis.Biochimica et biophysica acta · 2014Review
- The zinc-binding region (ZBR) fragment of Emi2 can inhibit APC/C by targeting its association with the coactivator Cdc20 and UBE2C-mediated ubiquitylation.FEBS open bio · 2014Article
- Modulation of cell cycle control during oocyte-to-embryo transitions.The EMBO journal · 2013Review
- EMI1, a three-in-one ubiquitylation inhibitor.Nature structural & molecular biology · 2013Article
- Electron microscopy structure of human APC/C(CDH1)-EMI1 reveals multimodal mechanism of E3 ligase shutdown.Nature structural & molecular biology · 2013Article
- Emi1 preferentially inhibits ubiquitin chain elongation by the anaphase-promoting complex.Nature cell biology · 2013Article
- OSD1 promotes meiotic progression via APC/C inhibition and forms a regulatory network with TDM and CYCA1;2/TAM.PLoS genetics · 2012Article
- Arabidopsis ULTRAVIOLET-B-INSENSITIVE4 maintains cell division activity by temporal inhibition of the anaphase-promoting complex/cyclosome.The Plant cell · 2011Article
- The emerging role of APC/CCdh1 in development.Seminars in cell & developmental biology · 2011Review
- Regulation of the action of early mitotic inhibitor 1 on the anaphase-promoting complex/cyclosome by cyclin-dependent kinases.The Journal of biological chemistry · 2011Article
- State of the APC/C: organization, function, and structure.Critical reviews in biochemistry and molecular biology · 2011Review
Corrections and comments
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Authors and funding
9 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Emi2 (also called Erp1) inhibits the anaphase-promoting complex/cyclosome (APC/C) and thereby causes metaphase II arrest in unfertilized vertebrate eggs. Both the D-box and the zinc-binding region (ZBR) of Emi2 have been implicated in APC/C inhibition. However, it is not well known how Emi2 interacts with and hence inhibits the APC/C. Here we show that Emi2 binds the APC/C via the C-terminal tail, termed here the RL tail. When expressed in Xenopus oocytes and egg extracts, Emi2 lacking the RL tail fails to interact with and inhibit the APC/C. The RL tail itself can directly bind to the APC/C, and, when added to egg extracts, either an excess of RL tail peptides or anti-RL tail peptide antibody can dissociate endogenous Emi2 from the APC/C, thus allowing APC/C activation. Furthermore, and importantly, the RL tail-mediated binding apparently promotes the inhibitory interactions of the D-box and the ZBR (of Emi2) with the APC/C. Finally, Emi1, a somatic paralog of Emi2, also has a functionally similar RL tail. We propose that the RL tail of Emi1/Emi2 serves as a docking site for the APC/C, thereby promoting the interaction and inhibition of the APC/C by the D-box and the ZBR.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.