Evidence map›Paper›PMID 20089801›Full record

ArticleThe Journal of general virology2010

Identification of phosphorylated residues on varicella-zoster virus immediate-early protein ORF63.

Niklaus H Mueller, Matthew S Walters, Roland A Marcus, Laurie L Graf, Jessica Prenni, Don Gilden, Saul J Silverstein, Randall J Cohrs

Abstract read
In one paragraph

Article in The Journal of general virology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
0.8field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 13 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Niklaus H MuellerDepartment of Neurology, University of Colorado Denver School of Medicine, Denver, USA.
Matthew S Walters
Roland A Marcus
Laurie L Graf
Jessica Prenni
Don Gilden
Saul J Silverstein
Randall J Cohrs
University of Colorado Denver · USColumbia University · USColorado State University · US

Funding

VARICELLA ZOSTER VIRUS VASCULOPATHY: CHANGING CLINICAL PRACTICEP01AG032958 · NIA · UNIVERSITY OF COLORADO DENVER · PI COHRS, RANDALL J. · 2009 to 2023
$30.3M
Varicella Zoster Virus Latency in Human GangliaP01NS032623 · NINDS · UNIVERSITY OF COLORADO DENVER · PI MAHLAINGAM, RAVI · 1993 to 2008
$12.3M
NEUROBIOLOGY OF VARICELLA-ZOSTER VIRUSR01AG006127 · NIA · UNIVERSITY OF COLORADO DENVER · PI GILDEN, DONALD · 1987 to 2014
$4.2M
VZ VIRUS: CLINICAL IMPLICATIONS OF IMMUNITYR01AI024021 · NIAID · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI SILVERSTEIN, SAUL J · 1986 to 2008
$3.0M
NEUROVIROLOGY-MOLECULAR BIOLOGYT32NS007321 · NINDS · UNIVERSITY OF COLORADO DENVER · PI GILDEN, DONALD · 1988 to 2014
$2.3M
NEUROBIOLOGY OF VARICELLA ZOSTER VIRUSR37AG006127 · NIA · UNIVERSITY OF COLORADO DENVER · PI GILDEN, DONALD H · 1995 to 2004
$2.3M
Varicella Zoster Virus:Clinical Implications of ImmunityR21AI024021 · NIAID · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI SILVERSTEIN, SAUL J · 2003 to 2003
$363k
NIAID NIH HHS AI-024021NIAID NIH HHS R01 AI024021NIAID NIH HHS R21 AI024021NIA NIH HHS AG006127NIA NIH HHS AG032958NIA NIH HHS P01 AG032958NIA NIH HHS R01 AG006127NIA NIH HHS R37 AG006127NINDS NIH HHS NS07321NINDS NIH HHS NS32623NINDS NIH HHS P01 NS032623NINDS NIH HHS T32 NS007321
6 · The paper itself

Abstract

Efficient replication of varicella-zoster virus (VZV) in cell culture requires expression of protein encoded by VZV open reading frame 63 (ORF63p). Two-dimensional gel analysis demonstrates that ORF63p is extensively modified. Mass spectroscopy analysis of ORF63p isolated from transiently transfected HEK 293 and stably transfected MeWo cells identified 10 phosphorylated residues. In VZV-infected MeWo cells, only six phosphorylated residues were detected. This report identifies phosphorylation of two previously uncharacterized residues (Ser5 and Ser31) in ORF63p extracted from cells infected with VZV or transfected with an ORF63p expression plasmid. Computational analysis of ORF63p for known kinase substrates did not identify Ser5 or Ser31 as candidate phosphorylation sites, suggesting that either atypical recognition sequences or novel cellular kinases are involved in ORF63p post-translational modification.

Indexed as

Virus ReplicationAmino Acid SequenceCell LineElectrophoresis, Gel, Two-DimensionalHerpesvirus 3, HumanHumansImmediate-Early ProteinsMass SpectrometryMolecular Sequence DataPhosphorylationProtein Processing, Post-TranslationalViral Envelope Proteinsimmediate early protein 63, Human herpesvirus 3Immediate-Early ProteinsViral Envelope Proteins

Identifiers

PMID20089801
PMCPMC2888152
OpenAlexW2081198144

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.