ArticlePlant physiology2010
Reconstruction of metabolic pathways, protein expression, and homeostasis machineries across maize bundle sheath and mesophyll chloroplasts: large-scale quantitative proteomics using the first maize genome assembly.
Article in Plant physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 88 papers, 1 of them a synthesis that pooled it.
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Who cites it
88 citing papers in PubMed, 1 synthesis or guideline pooled it, 201 citations in OpenAlex.
- Freeze-quenched maize mesophyll and bundle sheath separation uncovers bias in previous tissue-specific RNA-Seq data.Journal of experimental botany · 2017Pooled it
- Coordinated regulation of amino acid metabolism and transport in cereal nitrogen remobilization for grain filling.Frontiers in plant science · 2026Review
- In vivo two-photon FLIM resolves photosynthetic properties of maize bundle sheath cells.Photosynthesis research · 2025Article
- Engineering Crassulacean Acid Metabolism in CCold Spring Harbor perspectives in biology · 2024Review
- Comparison of plastid proteomes points towards a higher plastidial redox turnover in vascular tissues than in mesophyll cells.Journal of experimental botany · 2023Article
- Comparative transcriptomics reveals the role of altered energy metabolism in the establishment of single-cell CFrontiers in plant science · 2023Article
- A Changing Light Environment Induces Significant Lateral COInternational journal of molecular sciences · 2022Article
- Gene co-expression reveals the modularity and integration of C4 and CAM in Portulaca.Plant physiology · 2022Article
- Network and epigenetic characterization of subsets of genes specifically expressed in maize bundle sheath cells.Computational and structural biotechnology journal · 2022Article
- Understanding Maize Response to Nitrogen Limitation in Different Light Conditions for the Improvement of Photosynthesis.Plants (Basel, Switzerland) · 2021Review
- Ribosome profiling elucidates differential gene expression in bundle sheath and mesophyll cells in maize.Plant physiology · 2021Article
- Evidence for phloem loading via the abaxial bundle sheath cells in maize leaves.The Plant cell · 2021Article
- Photosynthetic Linear Electron Flow Drives COInternational journal of molecular sciences · 2021Article
- The Significance of Chloroplast NAD(P)H Dehydrogenase Complex and Its Dependent Cyclic Electron Transport in Photosynthesis.Frontiers in plant science · 2021Review
- Using single-plant-omics in the field to link maize genes to functions and phenotypes.Molecular systems biology · 2020Article
- The Phosphoglycerate Kinase (PGK) Gene Family of Maize (Plants (Basel, Switzerland) · 2020Article
- Autocatalytic Processing and Substrate Specificity of Arabidopsis Chloroplast Glutamyl Peptidase.Plant physiology · 2020Article
- Genetic determinants controlling maize rubisco activase gene expression and a comparison with rice counterparts.BMC plant biology · 2019Article
- The Enigmatic Roles of PPR-SMR Proteins in Plants.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Review
- Leveraging GWAS data to identify metabolic pathways and networks involved in maize lipid biosynthesis.The Plant journal : for cell and molecular biology · 2019Article
28 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chloroplasts in differentiated bundle sheath (BS) and mesophyll (M) cells of maize (Zea mays) leaves are specialized to accommodate C(4) photosynthesis. This study provides a reconstruction of how metabolic pathways, protein expression, and homeostasis functions are quantitatively distributed across BS and M chloroplasts. This yielded new insights into cellular specialization. The experimental analysis was based on high-accuracy mass spectrometry, protein quantification by spectral counting, and the first maize genome assembly. A bioinformatics workflow was developed to deal with gene models, protein families, and gene duplications related to the polyploidy of maize; this avoided overidentification of proteins and resulted in more accurate protein quantification. A total of 1,105 proteins were assigned as potential chloroplast proteins, annotated for function, and quantified. Nearly complete coverage of primary carbon, starch, and tetrapyrole metabolism, as well as excellent coverage for fatty acid synthesis, isoprenoid, sulfur, nitrogen, and amino acid metabolism, was obtained. This showed, for example, quantitative and qualitative cell type-specific specialization in starch biosynthesis, arginine synthesis, nitrogen assimilation, and initial steps in sulfur assimilation. An extensive overview of BS and M chloroplast protein expression and homeostasis machineries (more than 200 proteins) demonstrated qualitative and quantitative differences between M and BS chloroplasts and BS-enhanced levels of the specialized chaperones ClpB3 and HSP90 that suggest active remodeling of the BS proteome. The reconstructed pathways are presented as detailed flow diagrams including annotation, relative protein abundance, and cell-specific expression pattern. Protein annotation and identification data, and projection of matched peptides on the protein models, are available online through the Plant Proteome Database.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.