ArticleRNA (New York, N.Y.)2010
The RNA strands of the plus and minus polarities of peach latent mosaic viroid fold into different structures.
Article in RNA (New York, N.Y.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed.
- A scenario for the emergence of protoviroids in the RNA world and for their further evolution into viroids and viroid-like RNAs by modular recombinations and mutations.Virus evolution · 2022Review
- Direct visualization of the native structure of viroid RNAs at single-molecule resolution by atomic force microscopy.RNA biology · 2019Article
- Interference between variants of peach latent mosaic viroid reveals novel features of its fitness landscape: implications for detection.Scientific reports · 2017Article
- Article
- Elucidation of the structures of all members of the Avsunviroidae family.Molecular plant pathology · 2014Article
- Structural analyses of Avocado sunblotch viroid reveal differences in the folding of plus and minus RNA strands.Viruses · 2014Article
- The use of a combination of computer-assisted structure prediction and SHAPE probing to elucidate the secondary structures of five viroids.Molecular plant pathology · 2012Article
- Viroids: from genotype to phenotype just relying on RNA sequence and structural motifs.Frontiers in microbiology · 2012Article
- Mapping studies of the Peach latent mosaic viroid reveal novel structural features.Molecular plant pathology · 2011Article
- Replication of avocado sunblotch viroid in the yeast Saccharomyces cerevisiae.Journal of virology · 2011Article
- In-depth sequencing of the siRNAs associated with peach latent mosaic viroid infection.BMC molecular biology · 2010Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
It is believed that peach latent mosaic viroid (PLMVd) strands of both the plus and minus polarities fold into similar secondary and tertiary structures. In order to verify this hypothesis, the behavior of both strands in three biophysical assays was examined. PLMVd transcripts of plus and minus polarity were found to exhibit distinct electrophoretic mobility properties under native conditions, to precipitate differently in the presence of lithium chloride, and to possess variable thermal denaturation profiles. Subsequently, the structure of PLMVd transcripts of minus polarity was elucidated by biochemical methods, thereby permitting comparison to the known structure of the plus polarity. Specifically, enzymatic probing, electrophoretic mobility shift assay, and ribonuclease H hydrolysis were performed in order to resolve the secondary structure of the minus polarity. The left domains of the strands of both polarities appear to be similar, while the right domain exhibited several differences even though they both adopted a branched structure. The pseudoknot P8 formed in the plus strand seemed not formed in the minus strands. The structural differences between the two polarities might have important implications in various steps of the PLMVd life cycle.
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