ArticleJournal of the American Society for Mass Spectrometry2010
Nonspecific interactions between proteins and charged biomolecules in electrospray ionization mass spectrometry.
Article in Journal of the American Society for Mass Spectrometry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 51 citations in OpenAlex.
- Perturbation-Modulated Native Mass Spectrometry Excludes a Nonspecific Drug Target Protein Binder Based on Conformation Stability Change.Analytical chemistry · 2025Article
- Fragment-based drug discovery campaigns guided by native mass spectrometry.RSC medicinal chemistry · 2024Review
- A druggable conformational switch in the c-MYC transactivation domain.Nature communications · 2024Article
- Towards better understanding of the heparin role in NETosis: feasibility of using native mass spectrometry to monitor interactions of neutrophil elastase with heparin oligomers.International journal of mass spectrometry · 2021Article
- Native Mass Spectrometry of Membrane Proteins.Analytical chemistry · 2021Review
- CUPRA-ZYME: An Assay for Measuring Carbohydrate-Active Enzyme Activities, Pathways, and Substrate Specificities.Analytical chemistry · 2020Article
- Imidazole Derivatives Improve Charge Reduction and Stabilization for Native Mass Spectrometry.Analytical chemistry · 2019Article
- Insights into histidine kinase activation mechanisms from the monomeric blue light sensor EL346.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Quantifying the binding stoichiometry and affinity of histo-blood group antigen oligosaccharides for human noroviruses.Glycobiology · 2018Article
- Optimization of Electrospray Ionization by Statistical Design of Experiments and Response Surface Methodology: Protein-Ligand Equilibrium Dissociation Constant Determinations.Journal of the American Society for Mass Spectrometry · 2016Article
- Probing allosteric mechanisms using native mass spectrometry.Current opinion in structural biology · 2015Review
- Determination of Noncovalent Binding Using a Continuous Stirred Tank Reactor as a Flow Injection Device Coupled to Electrospray Ionization Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2015Article
- Quantifying protein-carbohydrate interactions using liquid sample desorption electrospray ionization mass spectrometry.Journal of the American Society for Mass Spectrometry · 2015Article
- Gangliosides are ligands for human noroviruses.Journal of the American Chemical Society · 2014Article
- Boundaries of mass resolution in native mass spectrometry.Journal of the American Society for Mass Spectrometry · 2014Article
- High throughput screening of high-affinity ligands for proteins with anion-binding sites using desorption electrospray ionization (DESI) mass spectrometry.Journal of the American Society for Mass Spectrometry · 2014Article
- Affinities of recombinant norovirus P dimers for human blood group antigens.Glycobiology · 2013Article
- Improved accuracy of low affinity protein-ligand equilibrium dissociation constants directly determined by electrospray ionization mass spectrometry.Journal of the American Society for Mass Spectrometry · 2012Article
- Reliable determinations of protein-ligand interactions by direct ESI-MS measurements. Are we there yet?Journal of the American Society for Mass Spectrometry · 2012Article
- Complexes of the outer mitochondrial membrane protein mitoNEET with resveratrol-3-sulfate.Biochemistry · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An investigation of the nonspecific association of small charged biomolecules and proteins in electrospray ionization mass spectrometry (ES-MS) is described. Aqueous solutions containing pairs of proteins and a small acidic or basic biomolecule that does not interact specifically with either of the proteins were analyzed by ES-MS and the distributions of the biomolecules bound nonspecifically to each pair of proteins compared. For the basic amino acid arginine and the peptide RGVFRR, nonequivalent distributions were measured in positive ion mode, but equivalent distributions were measured in negative ion mode. In the case of uridine 5'-diphosphate, nonequivalent distributions were measured in negative ion mode, but equivalent distributions observed in positive ion mode. The results of dissociation experiments performed on the gaseous ions of the nonspecific complexes suggest that the nonequivalent distributions result from differences in the extent to which the nonspecific complexes undergo in-source dissociation. To test this hypothesis, the distributions of nonspecifically bound basic molecules measured in the presence of imidazole, which protects complexes from in-source dissociation, were compared. In all cases, equivalent distributions were obtained. The results indicate that nonspecific binding of charged molecules to proteins during ES is a statistical process, independent of protein structure and size. However, the kinetic stabilities of the nonspecific interactions are sensitive to the nature of the protein ions. It is concluded that the reference protein method for correcting ES mass spectra for nonspecific ligand-protein binding can be applied to the analysis of ionic ligands, provided that in-source dissociation of the nonspecific interactions is minimized.
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