ArticleBMC microbiology2010
Metabolites produced by probiotic Lactobacilli rapidly increase glucose uptake by Caco-2 cells.
Article in BMC microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 43 citations in OpenAlex.
- Four weeks of probiotic supplementation reduces GI symptoms during a marathon race.European journal of applied physiology · 2019Trial
- Article
- Probiotic Modulation of the Gut-Ovary and Gut-Myometrium Axes: An In Vitro Study.Microorganisms · 2026Article
- Mathematical Modeling ofInternational journal of molecular sciences · 2025Article
- Prevention and treatment of antibiotics-associated adverse effects through the use of probiotics: A review.Journal of advanced research · 2025Review
- The Gut Microbiota-Related Antihyperglycemic Effect of Metformin.Pharmaceuticals (Basel, Switzerland) · 2025Review
- [Research progress on the mechanisms of probiotics promoting wound healing].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2024Review
- Prodigiosin-Functionalized Probiotic Ghosts as a Bioinspired Combination Against Colorectal Cancer Cells.Probiotics and antimicrobial proteins · 2023Article
- The Potential Value of Probiotics after Dental Implant Placement.Microorganisms · 2023Review
- Unveiling the potential pleiotropic effects of metformin in treating COVID-19: a comprehensive review.Frontiers in molecular biosciences · 2023Review
- Metformin in therapeutic applications in human diseases: its mechanism of action and clinical study.Molecular biomedicine · 2022Review
- Probiotic Mechanisms Affecting Glucose Homeostasis: A Scoping Review.Life (Basel, Switzerland) · 2022Article
- Intestinal microbiota and its interaction to intestinal health in nursery pigs.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2022Review
- The Relationship between the Gut Microbiome and Metformin as a Key for Treating Type 2 Diabetes Mellitus.International journal of molecular sciences · 2021Review
- Antihyperglycemic effect of rice husk derived xylooligosaccharides in high-fat diet and low-dose streptozotocin-induced type 2 diabetic rat model.Food science & nutrition · 2020Article
- Probiotics or pro-healers: the role of beneficial bacteria in tissue repair.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society · 2017Review
- Chloride secretion induced by rotavirus is oxidative stress-dependent and inhibited by Saccharomyces boulardii in human enterocytes.PloS one · 2014Article
- Glucose transporter 8 (GLUT8) regulates enterocyte fructose transport and global mammalian fructose utilization.Endocrinology · 2012Article
- Probiotics, nuclear receptor signaling, and anti-inflammatory pathways.Gastroenterology research and practice · 2011Article
- Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAlthough probiotic bacteria and their metabolites alter enterocyte gene expression, rapid, non-genomic responses have not been examined. The present study measured accumulation of tracer (2 microM) glucose by Caco-2 cells after exposure for 10 min or less to a chemically defined medium (CDM) with different monosaccharides before and after anaerobic culture of probiotic Lactobacilli.
resultsGrowth of L. acidophilus was supported by CDM with 110 mM glucose, fructose, and mannose, but not with arabinose, ribose, and xylose or the sugar-free CDM. Glucose accumulation was reduced when Caco-2 cells were exposed for 10 min to sterile CDM with glucose (by 92%), mannose (by 90%), fructose (by 55%), and ribose (by 16%), but not with arabinose and xylose. Exposure of Caco-2 cells for 10 min to bacteria-free supernatants prepared after exponential (48 h) and stationary (72 h) growth phases of L. acidophilus cultured in CDM with 110 mM fructose increased glucose accumulation by 83% and 45%, respectively; exposure to a suspension of the bacteria had no effect. The increase in glucose accumulation was diminished by heat-denaturing the supernatant, indicating the response of Caco-2 cells is triggered by as yet unknown heat labile bacterial metabolites, not by a reduction in CDM components that decrease glucose uptake. Supernatants prepared after anaerobic culture of L. gasseri, L. amylovorus, L. gallinarum, and L. johnsonii in the CDM with fructose increased glucose accumulation by 83%, 32%, 27%, and 14%, respectively.
conclusionThe rapid, non-genomic upregulation of SGLT1 by bacterial metabolites is a heretofore unrecognized interaction between probiotics and the intestinal epithelium.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.