ArticleBMC developmental biology2010
Analysis of retinal cell development in chick embryo by immunohistochemistry and in ovo electroporation techniques.
Article in BMC developmental biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 53 citations in OpenAlex.
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- Brosimine B and the biphasic dose-response: insights into hormesis and retinal neuroprotection.Frontiers in pharmacology · 2025Article
- Characterization of the development of the high-acuity area of the chick retina.Developmental biology · 2024Article
- Influence of Different Light Spectra on Melatonin Synthesis by the Pineal Gland and Influence on the Immune System in Chickens.Animals : an open access journal from MDPI · 2023Review
- Cell Fate of Retinal Progenitor Cells: In Ovo UbC-StarTrack Analysis.International journal of molecular sciences · 2022Article
- Unfolding a sequence of sensory influences and interactions in the development of functional brain laterality.Frontiers in behavioral neuroscience · 2022Review
- A single-plasmid approach for genome editing coupled with long-term lineage analysis in chick embryos.Development (Cambridge, England) · 2021Article
- The Appearance of the Warburg Effect in the Developing Avian Eye Characterized In Ovo: How Neurogenesis Can Remodel Neuroenergetics.Investigative ophthalmology & visual science · 2020Article
- Lineage tracing analysis of cone photoreceptor associated cis-regulatory elements in the developing chicken retina.Scientific reports · 2019Article
- Expression of the heparin-binding growth factors Midkine and pleiotrophin during ocular development.Gene expression patterns : GEP · 2019Article
- Whole genome DNA methylation sequencing of the chicken retina, cornea and brain.Scientific data · 2017Article
- RNA sequencing analysis of the developing chicken retina.Scientific data · 2016Article
- Horizontal cells expressing melanopsin x are novel photoreceptors in the avian inner retina.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
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- A regulatory sequence from theMolecular vision · 2016Article
- Identification of a transient Sox5 expressing progenitor population in the neonatal ventral forebrain by a novel cis-regulatory element.Developmental biology · 2014Article
- Expression of CXCL12 and CXCL14 during eye development in chick and mouse.Gene expression patterns : GEP · 2013Article
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- A cis-element in the Notch1 locus is involved in the regulation of gene expression in interneuron progenitors.Developmental biology · 2012Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundRetinal cell development has been extensively investigated; however, the current knowledge of dynamic morphological and molecular changes is not yet complete.
resultsThis study was aimed at revealing the dynamic morphological and molecular changes in retinal cell development during the embryonic stages using a new method of targeted retinal injection, in ovo electroporation, and immunohistochemistry techniques. A plasmid DNA that expresses the green fluorescent protein (GFP) as a marker was delivered into the sub-retinal space to transfect the chick retinal stem/progenitor cells at embryonic day 3 (E3) or E4 with the aid of pulses of electric current. The transfected retinal tissues were analyzed at various stages during chick development from near the start of neurogenesis at E4 to near the end of neurogenesis at E18. The expression of GFP allowed for clear visualization of cell morphologies and retinal laminar locations for the indication of retinal cell identity. Immunohistochemistry using cell type-specific markers (e.g., Visinin, Xap-1, Lim1+2, Pkcalpha, NeuN, Pax6, Brn3a, Vimentin, etc.) allowed further confirmation of retinal cell types. The composition of retinal cell types was then determined over time by counting the number of GFP-expressing cells observed with morphological characteristics specific to the various retinal cell types.
conclusionThe new method of retinal injection and electroporation at E3 - E4 allows the visualization of all retinal cell types, including the late-born neurons, e.g., bipolar cells at a level of single cells, which has been difficult with a conventional method with injection and electroporation at E1.5. Based on data collected from analyses of cell morphology, laminar locations in the retina, immunohistochemistry, and cell counts of GFP-expressing cells, the time-line and dynamic morphological and molecular changes of retinal cell development were determined. These data provide more complete information on retinal cell development, and they can serve as a reference for the investigations in normal retinal development and diseases.
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