ArticleNano letters2010
Nanopore based sequence specific detection of duplex DNA for genomic profiling.
Article in Nano letters, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
63 citing papers in PubMed, 178 citations in OpenAlex.
- Nanopore-based detection of circulating microRNAs in lung cancer patients.Nature nanotechnology · 2011Trial
- Article
- Decoding information stored in DNAs through single-molecule electrical detection platforms.Innovation (Cambridge (Mass.)) · 2025Review
- Chromosome defects and male factor infertility.Fertility and sterility · 2025Review
- Sensing the DNA-mismatch tolerance of catalytically inactive Cas9 via barcoded DNA nanostructures in solid-state nanopores.Nature biomedical engineering · 2024Article
- Single molecule technique unveils the role of electrostatic interactions in ssDNA-gp32 molecular complex stability.RSC advances · 2024Article
- DNA Barcodes Using a Dual Nanopore Device.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Study on the controllability of the fabrication of single-crystal silicon nanopores/nanoslits with a fast-stop ionic current-monitored TSWE method.Microsystems & nanoengineering · 2023Article
- Voltage controlled shutter regulates channel size and motion direction of protein aperture as durable nano-electric rectifier-----An opinion in biomimetic nanoaperture.Biomaterials · 2022Article
- Drilling accurate nanopores for biosensors by energetic multi-wall carbon nanotubes: a molecular dynamics investigation.Journal of molecular modeling · 2022Article
- Perspectives on conformationally constrained peptide nucleic acid (PNA): insights into the structural design, properties and applications.RSC chemical biology · 2022Review
- Digital immunoassay for biomarker concentration quantification using solid-state nanopores.Nature communications · 2021Article
- Single-Molecule Binding Assay Using Nanopores and Dimeric NP Conjugates.Advanced materials (Deerfield Beach, Fla.) · 2021Article
- DNA barcode by flossing through a cylindrical nanopore.RSC advances · 2021Article
- Article
- Sequence-Specific Detection of DNA Strands Using a Solid-State Nanopore Assisted by Microbeads.Micromachines · 2020Article
- Article
- Sequence-specific detection of single-stranded DNA with a gold nanoparticle-protein nanopore approach.Scientific reports · 2020Article
- Solid-state nanopore fabrication by automated controlled breakdown.Nature protocols · 2020Article
- Monolithic Fabrication of NPN/SiNAdvanced materials interfaces · 2019Article
3 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
We demonstrate a purely electrical method for the single-molecule detection of specific DNA sequences, achieved by hybridizing double-stranded DNA (dsDNA) with peptide nucleic acid (PNA) probes and electrophoretically threading the DNA through sub-5 nm silicon nitride pores. Bis-PNAs were used as the tagging probes in order to achieve high affinity and sequence specificity. Sequence detection is performed by reading the ion current traces of individual translocating DNA molecules, which display a characteristic secondary blockade level, absent in untagged molecules. The potential for barcoding DNA is demonstrated through nanopore analysis of once-tagged and twice-tagged DNA at different locations on the same genomic fragment. Our high-throughput, long-read length method can be used to identify key sequences embedded in individual DNA molecules, without the need for amplification or fluorescent/radio labeling. This opens up a wide range of possibilities in human genomics as well as in pathogen detection for fighting infectious diseases.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.