ArticleLipids2010
Isolation and characterization of a stress-dependent plastidial delta12 fatty acid desaturase from the Antarctic microalga Chlorella vulgaris NJ-7.
Article in Lipids, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 36 citations in OpenAlex.
- Functional expression of Δ12 fatty acid desaturase modulates thermoregulatory behaviour in Drosophila.Scientific reports · 2020Article
- Identification and analysis of the FAD gene family in walnuts (Juglans regia L.) based on transcriptome data.BMC genomics · 2020Article
- Article
- Characterization and engineering of a dual-function diacylglycerol acyltransferase in the oleaginous marine diatomBiotechnology for biofuels · 2018Article
- Engineering the Chloroplast Genome of Oleaginous Marine MicroalgaFrontiers in plant science · 2018Article
- TheFrontiers in plant science · 2017Article
- TheFrontiers in plant science · 2017Review
- Regulation of the cholesterol biosynthetic pathway and its integration with fatty acid biosynthesis in the oleaginous microalga Nannochloropsis oceanica.Biotechnology for biofuels · 2014Article
- Genetic variation of six desaturase genes in flax and their impact on fatty acid composition.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2013Article
- Isolation and characterization of fatty acid desaturase genes from peanut (Arachis hypogaea L.).Plant cell reports · 2011Article
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An acclimation to the changing physicochemical conditions and high amount of Delta(12)-unsaturated fatty acids of the Antarctic Chlorella vulgaris NJ-7 prompted us to speculate about the involvement of Delta(12)-fatty acid desaturases (FAD) in its adaptation to the extremely unfavorable ambience. A full-length cDNA sequence, designated CvFAD6, was isolated from C. vulgaris NJ-7 via RT-PCR and RACE methods. Sequence alignment showed that the gene was homologous to corresponding Delta(12)-FAD from other eukaryotes. Phylogenetic analysis showed that it was grouped with plastidial Delta(12)-FAD with conserved histidine boxes. Yeast cells transformed with a plasmid construct containing CvFAD6 coding region accumulated a considerable amount of linoleic acid (18:2Delta(9,12)), normally not present in wild-type yeast cells, suggesting that the isolated gene encodes a functional Delta(12) enzyme. The correlation between the accumulation of CvFAD6 and temperature has been examined by real time PCR. The analysis showed a constant expression of CvFAD6 from 25 to 15 degrees C whereas a fourfold increased from 25 to 4 degrees C. Moreover, CvFAD6 transcription was more sensitive to saline stress since a 20-fold increase at 6% NaCl was detected. Our data demonstrate that CvFAD6 is the enzyme responsible for the Delta(12) fatty acids desaturation involved in low temperature and high salinity acclimation for Antarctic C. vulgaris NJ-7.
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