ArticleDigestive diseases and sciences2010
Subjects with diarrhea-predominant IBS have increased rectal permeability responsive to tryptase.
Article in Digestive diseases and sciences, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 2 of them syntheses that pooled it.
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Who cites it
34 citing papers in PubMed, 2 syntheses or guidelines pooled it, 66 citations in OpenAlex.
- Evidence-Based Clinical Guidelines for Chronic Diarrhea 2023.Digestion · 2024Guideline
- Global research progress of visceral hypersensitivity and irritable bowel syndrome: bibliometrics and visualized analysis.Frontiers in pharmacology · 2023Pooled it
- Trial
- Duodenal acidification induces gastric relaxation and alters epithelial barrier function by a mast cell independent mechanism.Scientific reports · 2020Trial
- The Mast Cell-PAR2-TRP Axis: A Convergent Mechanism for Visceral Hypersensitivity Independent of Divergent Motility in IBS.Biomolecules · 2026Review
- Hereditary α-tryptasemia; a review of mechanisms linking α-tryptase gene dosage to intestinal homeostasis and immunopathology.Frontiers in allergy · 2026Review
- Beyond the "Master" Role in Allergy: Insights into Intestinal Mast Cell Plasticity and Gastrointestinal Diseases.Biomedicines · 2025Review
- Non allergic gastrointestinal manifestations of hereditary alpha-tryptasemia.Frontiers in allergy · 2025Review
- High FODMAP diet causes barrier loss via lipopolysaccharide-mediated mast cell activation.JCI insight · 2021Article
- Article
- Intestinal barrier dysfunction in irritable bowel syndrome: a systematic review.Therapeutic advances in gastroenterology · 2021Review
- Increasing Evidence That Irritable Bowel Syndrome and Functional Gastrointestinal Disorders Have a Microbial Pathogenesis.Frontiers in cellular and infection microbiology · 2020Review
- Role for diet in normal gut barrier function: developing guidance within the framework of food-labeling regulations.American journal of physiology. Gastrointestinal and liver physiology · 2019Review
- Protease Activated Receptor-2 Induces Immune Activation and Visceral Hypersensitivity in Post-infectious Irritable Bowel Syndrome Mice.Digestive diseases and sciences · 2019Article
- Regulation of intestinal permeability: The role of proteases.World journal of gastroenterology · 2017Review
- Pathophysiology, Evaluation, and Management of Chronic Watery Diarrhea.Gastroenterology · 2017Review
- Review
- Tryptase and Protease-Activated Receptor 2 Expression Levels in Irritable Bowel Syndrome.Gut and liver · 2016Article
- Inflammation in irritable bowel syndrome: Myth or new treatment target?World journal of gastroenterology · 2016Review
- The Role of Mast Cells in Irritable Bowel Syndrome.Gastroenterology research and practice · 2016Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsPatients with diarrhea-predominant irritable bowel syndrome (IBS-D) appear to have increased intestinal permeability; it has been suggested that activation of protease-activated receptor-2 (PAR-2) receptors is responsible for this alteration. The aims of this study are to evaluate (1) if rectal (large bowel) permeability is increased in IBS-D and (2) if tryptase plays a critical role in the altered permeability.
methodsRectal biopsies from 20 patients with IBS-D and 30 subjects without the condition (normal controls) were assessed for macromolecular permeability using horseradish peroxidase in Ussing chambers in the basal state and after addition of drugs to the basolateral side. Reverse-transcription polymerase chain reaction (RT-PCR) was performed using colonic biopsy tissues from patients with IBS-D and normal subjects.
resultsWhen tryptase was added to the basolateral (not mucosal) side of normal rectal biopsy tissues, permeability appeared to be proportional to the increase in tryptase concentration (P < 0.05) and was abolished by the addition of tryptase inhibitor (100 μM nafamostat; 1.568 ± 0.874 ng/2 h/mm(2) to 0.766 ± 0.661 ng/2 h/mm(2), n = 14, respectively, P < 0.01). Intestinal permeability in patients with IBS-D was significantly increased compared with controls (0.848 ± 0.0.600 ng/2 h/mm(2), n = 21, P < 0.01). Nafamostat significantly reduced the enhanced permeability in IBS-D (0.934 ± 0.589 ng/2 h/mm(2) to 0.247 ± 0.263 ng/2 h/mm(2), n = 14, respectively, P < 0.05). Transcription levels of PAR2 measured by RT-PCR did not differ between IBS-D and normal subjects.
conclusionTryptase seems to play an important role in the control of human colonic mucosal permeability, and enhanced tryptase activity was responsible for the increased permeability of rectal mucosa in IBS patients.
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