Evidence map›Paper›PMID 20086252›Full record

ArticleDiabetes care2010

Comparison of radioimmunoprecipitation with luciferase immunoprecipitation for autoantibodies to GAD65 and IA-2beta.

Peter D Burbelo, Hiroki Hirai, Alexandra T Issa, Albert Kingman, Ake Lernmark, Sten-A Ivarsson, Abner L Notkins, Michael J Iadarola

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Diabetes care, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
2.8field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 33 citations in OpenAlex.

  1. Predictive Autoantibodies Before the Diagnosis of Type I Diabetes in Adults.medRxiv : the preprint server for health sciences · 2026
    Article
  2. Article
  3. Article
  4. Article
  5. Birth and coming of age of islet autoantibodies.Clinical and experimental immunology · 2019
    Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. Luciferase immunoprecipitation systems for measuring antibodies in autoimmune and infectious diseases.Translational research : the journal of laboratory and clinical medicine · 2015
    Review
  11. Article
  12. Article
  13. Article
  14. Article
  15. Review
  16. Article
  17. Review
  18. Article
  19. Extraordinary antigenicity of the human Ro52 autoantigen.American journal of translational research · 2010
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Peter D BurbeloNeurobiology and Pain Therapeutics Section, Laboratory of Sensory Biology, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland, USA.
Hiroki Hirai
Alexandra T Issa
Albert Kingman
Ake Lernmark
Sten-A Ivarsson
Abner L Notkins
Michael J Iadarola
National Institute of Dental and Craniofacial Research · USLund University · SENational Institutes of Health · US

Funding

Insulin-Dependent Diabetes MellitusZIADE000423 · NIDCR · NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH · PI NOTKINS, ABNER L. · 2009 to 2020
$16.9M
Mechanisms of Pain and Immune ProcessesZIADE000724 · NIDCR · NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH · PI IADAROLA, MICHAEL J. · 2009 to 2012
$2.6M
Intramural NIH HHS
6 · The paper itself

Abstract

objectiveTo compare the sensitivity and specificity of luciferase immunoprecipitation (LIPS) with radioimmunoprecipitation (RIP) for the measurement of autoantibodies to the type 1 diabetes autoantigens glutamic acid decarboxylase 65 (GAD65) and insulinoma-associated protein (IA)-2beta. RESEARCH DESIGN AND

methodsSera from 49 type 1 diabetic patients and 100 nondiabetic control subjects from Diabetes Antibody Standardization Program 2007 were used to screen for autoantibodies to GAD65. An additional 200 type 1 diabetic patients and 200 nondiabetic control subjects were used to validate the GAD65 results and screen for autoantibodies to IA-2beta.

resultsLIPS showed equal sensitivity and specificity to RIP for detecting autoantibodies to GAD65 and IA-2beta. Receiver-operating characteristic analysis revealed that the detection of autoantibodies to GAD65 and IA-2beta by LIPS and RIP were not statistically different.

conclusionsThe LIPS assay does not require the use of radioisotopes or in vitro transcription/translation and is a practical alternative at the clinical level for the RIP assay.

Indexed as

AutoantibodiesDiabetes Mellitus, Type 1Glutamate DecarboxylaseHumansImmunoprecipitationLuciferasesRadioimmunoprecipitation AssayReceptor-Like Protein Tyrosine Phosphatases, Class 8Sensitivity and SpecificityAutoantibodiesGlutamate Decarboxylaseglutamate decarboxylase 2LuciferasesReceptor-Like Protein Tyrosine Phosphatases, Class 8

Identifiers

PMID20086252
PMCPMC2845022
OpenAlexW2171607554

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.