ArticleMolecular and cellular biology2010
Identification and analysis of the interaction between Edc3 and Dcp2 in Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
43 citing papers in PubMed, 62 citations in OpenAlex.
- Sequence-encoded autoinhibition couples mRNA decapping activity to phase separation.bioRxiv : the preprint server for biology · 2026Article
- RNA anchoring of Upf1 facilitates recruitment of Dcp2 in the NMD decapping complex.Nucleic acids research · 2025Article
- Med13 is required for efficient P-body recruitment and autophagic degradation of Edc3 following nitrogen starvation.Molecular biology of the cell · 2024Article
- Eukaryotic mRNA decapping factors: molecular mechanisms and activity.The FEBS journal · 2023Review
- Eukaryotic mRNA Decapping Activation.Frontiers in genetics · 2022Review
- Biomolecular condensates amplify mRNA decapping by biasing enzyme conformation.Nature chemical biology · 2021Article
- Mutations in genes encoding regulators of mRNA decapping and translation initiation: links to intellectual disability.Biochemical Society transactions · 2020Review
- A quantitative inventory of yeast P body proteins reveals principles of composition and specificity.eLife · 2020Article
- Pby1 is a direct partner of the Dcp2 decapping enzyme.Nucleic acids research · 2020Article
- General decapping activators target different subsets of inefficiently translated mRNAs.eLife · 2018Article
- mRNA decapping: finding the right structures.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- Structure of the activated Edc1-Dcp1-Dcp2-Edc3 mRNA decapping complex with substrate analog poised for catalysis.Nature communications · 2018Article
- Intrinsically Disordered Regions Can Contribute Promiscuous Interactions to RNP Granule Assembly.Cell reports · 2018Article
- Numerous interactions act redundantly to assemble a tunable size of P bodies inProceedings of the National Academy of Sciences of the United States of America · 2017Article
- A unique surface on Pat1 C-terminal domain directly interacts with Dcp2 decapping enzyme and Xrn1 5'-3' mRNA exonuclease in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- A synergistic network of interactions promotes the formation of in vitro processing bodies and protects mRNA against decapping.Nucleic acids research · 2017Article
- The ApaH-like phosphatase TbALPH1 is the major mRNA decapping enzyme of trypanosomes.PLoS pathogens · 2017Article
- New insights into decapping enzymes and selective mRNA decay.Wiley interdisciplinary reviews. RNA · 2017Review
- Review
- Structure of the active form of Dcp1-Dcp2 decapping enzyme bound to mNature structural & molecular biology · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Cap hydrolysis is a critical control point in the life of eukaryotic mRNAs and is catalyzed by the evolutionarily conserved Dcp1-Dcp2 complex. In Saccharomyces cerevisiae, decapping is modulated by several factors, including the Lsm family protein Edc3, which directly binds to Dcp2. We show that Edc3 binding to Dcp2 is mediated by a short peptide sequence located C terminal to the catalytic domain of Dcp2. This sequence is required for Edc3 to stimulate decapping activity of Dcp2 in vitro, for Dcp2 to efficiently accumulate in P-bodies, and for efficient degradation of the RPS28B mRNA, whose decay is enhanced by Edc3. In contrast, degradation of YRA1 pre-mRNA, another Edc3-regulated transcript, occurs independently from this region, suggesting that the effect of Edc3 on YRA1 is independent of its interaction with Dcp2. Deletion of the sequence also results in a subtle but significant defect in turnover of the MFA2pG reporter transcript, which is not affected by deletion of EDC3, suggesting that the region affects some other aspect of Dcp2 function in addition to binding Edc3. These results raise a model for Dcp2 recruitment to specific mRNAs where regions outside the catalytic core promote the formation of different complexes involved in mRNA decapping.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.