ArticleInfection and immunity2010
Haemophilus ducreyi SapA contributes to cathelicidin resistance and virulence in humans.
Article in Infection and immunity, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 42 citations in OpenAlex.
- Molecular pathogenesis ofMicrobiology and molecular biology reviews : MMBR · 2025Review
- Understanding the Dynamics of Human Defensin Antimicrobial Peptides: Pathogen Resistance and Commensal Induction.Applied biochemistry and biotechnology · 2024Review
- Molecular Mechanisms of Bacterial Resistance to Antimicrobial Peptides in the Modern Era: An Updated Review.Microorganisms · 2024Review
- AInfection and immunity · 2024Article
- Antimicrobial Peptide Recognition Motif of the Substrate Binding Protein SapA from NontypeableBiochemistry · 2024Article
- Bacterial resistance to antibacterial agents: Mechanisms, control strategies, and implications for global health.The Science of the total environment · 2023Review
- The role of bacterial transport systems in the removal of host antimicrobial peptides in Gram-negative bacteria.FEMS microbiology reviews · 2022Review
- The Potential of Human Peptide LL-37 as an Antimicrobial and Anti-Biofilm Agent.Antibiotics (Basel, Switzerland) · 2021Review
- The Majority of Typhoid Toxin-PositivemSphere · 2021Article
- Article
- Interactions of the Skin PathogenFrontiers in immunology · 2020Review
- Article
- Selective substrate uptake: The role of ATP-binding cassette (ABC) importers in pathogenesis.Biochimica et biophysica acta. Biomembranes · 2018Review
- The SapA Protein Is Involved in Resistance to Antimicrobial Peptide PR-39 and Virulence ofFrontiers in microbiology · 2017Article
- Bacterial Evasion of Host Antimicrobial Peptide Defenses.Microbiology spectrum · 2016Review
- On the in vivo significance of bacterial resistance to antimicrobial peptides.Biochimica et biophysica acta · 2015Review
- Phosphoethanolamine Transferase LptA in Haemophilus ducreyi Modifies Lipid A and Contributes to Human Defensin Resistance In Vitro.PloS one · 2015Article
- Activation of CpxRA in Haemophilus ducreyi primarily inhibits the expression of its targets, including major virulence determinants.Journal of bacteriology · 2013Article
- Carbon storage regulator A contributes to the virulence of Haemophilus ducreyi in humans by multiple mechanisms.Infection and immunity · 2013Article
- Permeases of the sap transporter are required for cathelicidin resistance and virulence of Haemophilus ducreyi in humans.The Journal of infectious diseases · 2012Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
Haemophilus ducreyi is an extracellular pathogen of human epithelial surfaces that resists human antimicrobial peptides (APs). The organism's genome contains homologs of genes sensitive to antimicrobial peptides (sap operon) in nontypeable Haemophilus influenzae. In this study, we characterized the sap-containing loci of H. ducreyi 35000HP and demonstrated that sapA is expressed in broth cultures and H. ducreyi-infected tissue; sapA is also conserved among both class I and class II H. ducreyi strains. We constructed a nonpolar sapA mutant of H. ducreyi 35000HP, designated 35000HPsapA, and compared the percent survival of wild-type 35000HP and 35000HPsapA exposed to several human APs, including alpha-defensins, beta-defensins, and the cathelicidin LL-37. Unlike an H. influenzae sapA mutant, strain 35000HPsapA was not more susceptible to defensins than strain 35000HP was. However, we observed a significant decrease in the survival of strain 35000HPsapA after exposure to LL-37, which was complemented by introducing sapA in trans. Thus, the Sap transporter plays a role in resistance of H. ducreyi to LL-37. We next compared mutant strain 35000HPsapA with strain 35000HP for their ability to cause disease in human volunteers. Although both strains caused papules to form at similar rates, the pustule formation rate at sites inoculated with 35000HPsapA was significantly lower than that of sites inoculated with 35000HP (33.3% versus 66.7%; P = 0.007). Together, these data establish that SapA acts as a virulence factor and as one mechanism for H. ducreyi to resist killing by antimicrobial peptides. To our knowledge, this is the first demonstration that an antimicrobial peptide resistance mechanism contributes to bacterial virulence in humans.
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