ArticleInfection and immunity2010
Polysaccharide capsule and sialic acid-mediated regulation promote biofilm-like intracellular bacterial communities during cystitis.
Article in Infection and immunity, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
65 citing papers in PubMed, 128 citations in OpenAlex.
- Wzi modulates immune evasion of hypervirulentVirulence · 2026Article
- Bacterial strategies for surviving antibiotics during urinary tract infection: could uropathogenic Escherichia coli be persisters?Nature reviews. Urology · 2026Review
- UropathogenicAntibiotics (Basel, Switzerland) · 2026Review
- Genetic control of Group 3 (K96) capsule synthesis and complement resistance in extraintestinal pathogenicmSphere · 2026Article
- NeuO-mediated O-acetylation of uropathogenicJournal of bacteriology · 2026Article
- Capsule Regulation ShapesInternational journal of molecular sciences · 2026Article
- Genetic requirements for uropathogenicmSystems · 2024Article
- Uropathogen and host responses in pyelonephritis.Nature reviews. Nephrology · 2023Review
- Genetic Engineering of Bacteriophage K1F with Human Epidermal Growth Factor to Enhance Killing of IntracellularACS synthetic biology · 2023Article
- Evolutionary and functional history of the Escherichia coli K1 capsule.Nature communications · 2023Article
- Identification of novel genes involved in the biofilm formation process of Avian Pathogenic Escherichia coli (APEC).PloS one · 2022Article
- Regulation ofFrontiers in microbiology · 2022Review
- Improved methods to characterize the length and quantity of highly unstable PolySialic acids subject category: (Carbohydrates, chromatographic techniques).Analytical biochemistry · 2021Article
- The Short-Chain Fatty Acids Propionate and Butyrate Augment Adherent-Invasive Escherichia coli Virulence but Repress Inflammation in a Human Intestinal Enteroid Model of Infection.Microbiology spectrum · 2021Article
- Bile Salts Regulate Zinc Uptake and Capsule Synthesis in a Mastitis-Associated Extraintestinal Pathogenic Escherichia coli Strain.Infection and immunity · 2021Article
- The glycobiology of uropathogenic E. coli infection: the sweet and bitter role of sugars in urinary tract immunity.Immunology · 2021Review
- Alternative Therapeutic Options to Antibiotics for the Treatment of Urinary Tract Infections.Frontiers in microbiology · 2020Review
- The role of major virulence factors and pathogenicity of adherent-invasivePrzeglad gastroenterologiczny · 2020Review
- LysR-type transcriptional regulator OvrB encoded in O island 9 drives enterohemorrhagicVirulence · 2019Article
- The Autotransporter IcsA Promotes Shigella flexneri Biofilm Formation in the Presence of Bile Salts.Infection and immunity · 2019Article
5 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Uropathogenic Escherichia coli (UPEC) is the leading cause of urinary tract infections (UTIs). A murine UTI model has revealed an infection cascade whereby UPEC undergoes cycles of invasion of the bladder epithelium, intracellular proliferation in polysaccharide-containing biofilm-like masses called intracellular bacterial communities (IBC), and then dispersal into the bladder lumen to initiate further rounds of epithelial colonization and invasion. We predicted that the UPEC K1 polysaccharide capsule is a key constituent of the IBC matrix. Compared to prototypic E. coli K1 strain UTI89, a capsule assembly mutant had a fitness defect in functionally TLR4(+) and TLR4(-) mice, suggesting a protective role of capsule in inflamed and noninflamed hosts. K1 capsule assembly and synthesis mutants had dramatically reduced IBC formation, demonstrating the common requirement for K1 polysaccharide in IBC development. The capsule assembly mutant appeared dispersed in the cytoplasm of the bladder epithelial cells and failed to undergo high-density intracellular replication during later stages of infection, when the wild-type strain continued to form serial generations of IBC. Deletion of the sialic acid regulator gene nanR partially restored IBC formation in the capsule assembly mutant. These data suggest that capsule is necessary for efficient IBC formation and that aberrant sialic acid accumulation, resulting from disruption of K1 capsule assembly, produces a NanR-mediated defect in intracellular proliferation and IBC development. Together, these data demonstrate the complex but important roles of UPEC polysaccharide encapsulation and sialic acid signaling in multiple stages of UTI pathogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.