ArticleInfection and immunity2010
Screening of predicted secreted antigens from Mycobacterium bovis reveals the immunodominance of the ESAT-6 protein family.
Article in Infection and immunity, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 57 citations in OpenAlex.
- Conserved immune recognition hierarchy of mycobacterial PE/PPE proteins during infection in natural hosts.PloS one · 2012Trial
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- A molecularly defined skin test reagent for the diagnosis of bovine tuberculosis compatible with vaccination against Johne's Disease.Scientific reports · 2021Article
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- Identifying Bacterial and Host Factors Involved in the Interaction ofFrontiers in immunology · 2021Article
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- Immunogenicity and Vaccine Potential of InsB, an ESAT-6-Like Antigen Identified in the Highly VirulentFrontiers in microbiology · 2019Article
- Development and evaluation of an interferon gamma assay for the diagnosis of tuberculosis in red deer experimentally infected with Mycobacterium bovis.BMC veterinary research · 2017Article
- Mycobacterium tuberculosis HspX/EsxS Fusion Protein: Gene Cloning, Protein Expression, and Purification in Escherichia coli.Reports of biochemistry & molecular biology · 2017Article
- A novel multivalent tuberculosis vaccine confers protection in a mouse model of tuberculosis.Human vaccines & immunotherapeutics · 2016Article
- Integrated computational prediction and experimental validation identifies promiscuous T cell epitopes in the proteome ofMicrobial genomics · 2016Article
- Highly purified mycobacterial phosphatidylinositol mannosides drive cell-mediated responses and activate NKT cells in cattle.Clinical and vaccine immunology : CVI · 2015Article
- The role of badgers in the epidemiology ofVeterinary medicine (Auckland, N.Z.) · 2015Review
- Structural conservation, variability, and immunogenicity of the T6 backbone pilin of serotype M6 Streptococcus pyogenes.Infection and immunity · 2014Article
- Exploration of novel cellular and serological antigen biomarkers in the ORFeome of Mycobacterium tuberculosis.Molecular & cellular proteomics : MCP · 2014Article
- Mycobacterium tuberculosis as a sarcoid factor? A case report of family sarcoidosis.The American journal of case reports · 2014Article
- Function and Potentials of M. tuberculosis Epitopes.Frontiers in immunology · 2014Review
- Evaluation of cocktails with recombinant proteins of Mycobacterium bovis for a specific diagnosis of bovine tuberculosis.BioMed research international · 2014Article
- Multivalent TB vaccines targeting the esx gene family generate potent and broad cell-mediated immune responses superior to BCG.Human vaccines & immunotherapeutics · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Results of previous studies utilizing bioinformatic approaches in antigen-mining experiments revealed that secreted proteins are among the most frequently recognized antigens from Mycobacterium bovis. Thus, we hypothesized that the analysis of secreted proteins is likely to reveal additional immunogenic antigens that can be used to increase the specificity of diagnostic tests or be suitable vaccination candidates for mycobacterial infections. To test this hypothesis, 382 pools of overlapping peptides spanning 119 M. bovis secreted and potentially secreted proteins were screened for the ability to stimulate a gamma interferon response in vitro using whole blood from tuberculin-positive reactor (TB reactor) cattle. Of the 119 proteins screened, 70 (59%) induced positive responses in the TB reactor animals to various degrees. Strikingly, all but one of the 15 ESAT-6 proteins tested were recognized by at least 30% of the TB reactor animals, with 12 of the 22 most commonly recognized antigens belonging to this protein family. Further analysis of these data demonstrated that there was no significant difference in immunogenicity between the ESAT-6 proteins that were components of potentially intact ESX secretory systems and those corresponding to additional partial esx loci. Importantly for vaccine design, antigenic epitopes in some highly conserved regions shared by numerous ESAT-6 proteins were identified. However, despite this considerable homology, peptide-mapping experiments also revealed that immunodominant peptides were located in regions of amino acid variability.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.