ArticleRNA (New York, N.Y.)2010
Whole mount in situ hybridization detection of mRNAs using short LNA containing DNA oligonucleotide probes.
Article in RNA (New York, N.Y.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 32 citations in OpenAlex.
- Diacylglycerol kinase γ facilitates the proliferation and migration of neural stem cells in the developing neural tube.Acta biochimica et biophysica Sinica · 2024Article
- Progress of CRISPR-Cas13 Mediated Live-Cell RNA Imaging and Detection of RNA-Protein Interactions.Frontiers in cell and developmental biology · 2022Review
- Small RNA In Situ Hybridizations on Sections of Arabidopsis Embryos.Methods in molecular biology (Clifton, N.J.) · 2020Article
- PICS: a platform for planar imaging of curved surfaces of brain and other tissue.Brain structure & function · 2019Article
- Identification and Expression of Neurotrophin-6 in the Brain of Nothobranchius furzeri: One More Piece in Neurotrophin Research.Journal of clinical medicine · 2019Article
- Probing cytoplasmic and nuclear microRNAs in single living cellsChemical science · 2018Article
- N1-Src Kinase Is Required for Primary Neurogenesis inThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Recent advances in high-performance fluorescent and bioluminescent RNA imaging probes.Chemical Society reviews · 2017Review
- Quantitative measurement of cancer tissue biomarkers in the lab and in the clinic.Laboratory investigation; a journal of technical methods and pathology · 2015Review
- LNA-based in situ hybridization detection of mRNAs in embryos.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Cryptorchidism and infertility in rats with targeted disruption of the Adamts16 locus.PloS one · 2014Article
- LNA probes substantially improve the detection of bacterial endosymbionts in whole mount of insects by fluorescent in-situ hybridization.BMC microbiology · 2012Article
- A sensitive alternative for microRNA in situ hybridizations using probes of 2'-O-methyl RNA + LNA.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
In situ hybridization is widely used to visualize transcribed sequences in embryos, tissues, and cells. For whole mount detection of mRNAs in embryos, hybridization with an antisense RNA probe is followed by visual or fluorescence detection of target mRNAs. A limitation of this approach is that a cDNA template of the target RNA must be obtained in order to generate the antisense RNA probe. Here we investigate the use of short (12-24 nucleotides) locked nucleic acid (LNA) containing DNA probes for whole mount in situ hybridization detection of mRNAs. Following extensive protocol optimization, we show that LNA probes can be used to localize several mRNAs of varying abundances in chicken embryos. LNA probes also detected alternatively spliced exons that are processed in a tissue specific manner. The use of LNA probes for whole mount in situ detection of mRNAs will enable in silico design and chemical synthesis and will expand the general use of in situ hybridization for studies of transcriptional regulation and alternative splicing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.