ArticleMethods (San Diego, Calif.)2010
High resolution melting analysis for gene scanning.
Article in Methods (San Diego, Calif.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
57 citing papers in PubMed, 2 syntheses or guidelines pooled it, 166 citations in OpenAlex.
- Diagnostic accuracy of high-resolution melting curve analysis for discrimination of oncology-associatedThe Journal of international medical research · 2025Pooled it
- High resolution melting assay as a reliable method for diagnosing drug-resistant TB cases: a systematic review and meta-analysis.BMC infectious diseases · 2021Pooled it
- Impaired Motor Function in a Zebrafish Stathmin-2 Knockout Model.Developmental neurobiology · 2026Article
- Analytical validation of a high-resolution melting assay for UGT1A1 TATA-box polymorphisms.Molecular biology reports · 2026Article
- Development and Validation of a High-Resolution Melting (HRM) Method for Differentiating Ovis and Equi Biovars ofVeterinary sciences · 2026Article
- Species Identification of Sea Turtles in Thailand Using DNA Barcoding Coupled With High-Resolution Melting (Bar-HRM) Analysis.Scientifica · 2026Article
- Identification of lineage-associated polymorphisms in the ROP18 3' flanking region and development of molecular assays for differentiation ofFrontiers in microbiology · 2026Article
- When DNA Tells the Tale: High-Resolution Melting as a Forensic Tool for Mediterranean Cetacean Identification.International journal of molecular sciences · 2025Article
- Development of a Multi-Locus Real-Time PCR with a High-Resolution Melting Assay to Differentiate Wild-Type, Asian Recombinant, and Vaccine Strains of Lumpy Skin Disease Virus.Veterinary sciences · 2025Article
- Simultaneous detection and differentiation of classical Muscovy duck reovirus and goose-origin Muscovy duck reovirus by RT-qPCR assay with high-resolution melting analysis.Frontiers in veterinary science · 2024Article
- Article
- Congenital Dyserythropoietic Anemia Type II: High Prevalence of c.1385A>G, (p.Tyr462Cys) Mutation in the Indian Population.Indian journal of pediatrics · 2023Article
- Development of High-Resolution Melting Curve Analysis for rapid detection of SEC23B gene mutation causing Congenital Dyserythropoietic Anemia type II in Indian population.Italian journal of pediatrics · 2023Article
- Genetic analysis of ACE2 peptidase domain in SARS-CoV-2-positive and SARS-CoV-2-negative individuals from Pakistan.Molecular biology reports · 2023Article
- Discrimination of human papillomavirus genotypes using innovative technique nested-high resolution melting.Scientific reports · 2022Article
- Genome-wide analysis identified novel susceptible genes of restless legs syndrome in migraineurs.The journal of headache and pain · 2022Article
- A high-resolution melting assay to differentiate a peste des petits ruminants virus vaccine strain from field isolates in Turkey.Archives of virology · 2022Article
- Development of high-resolution melting (HRM) assay to differentiate the species of Shigella isolates from stool and food samples.Scientific reports · 2022Article
- Molecular Genetic Techniques in Biomarker Analysis Relevant for Drugs Centrally Approved in Europe.Molecular diagnosis & therapy · 2022Review
- Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
High resolution melting is a new method of genotyping and variant scanning that can be seamlessly appended to PCR amplification. Limitations of genotyping by amplicon melting can be addressed by unlabeled probe or snapback primer analysis, all performed without labeled probes. High resolution melting can also be used to scan for rare sequence variants in large genes with multiple exons and is the focus of this article. With the simple addition of a heteroduplex-detecting dye before PCR, high resolution melting is performed without any additions, processing or separation steps. Heterozygous variants are identified by atypical melting curves of a different shape compared to wild-type homozygotes. Homozygous or hemizygous variants are detected by prior mixing with wild-type DNA. Design, optimization, and performance considerations for high resolution scanning assays are presented for rapid turnaround of gene scanning. Design concerns include primer selection and predicting melting profiles in silico. Optimization includes temperature gradient selection of the annealing temperature, random population screening for common variants, and batch preparation of primer plates with robotically deposited and dried primer pairs. Performance includes rapid DNA preparation, PCR, and scanning by high resolution melting that require, in total, only 3h when no variants are present. When variants are detected, they can be identified in an additional 3h by rapid cycle sequencing and capillary electrophoresis. For each step in the protocol, a general overview of principles is provided, followed by an in depth analysis of one example, scanning of CYBB, the gene that is mutated in X-linked chronic granulomatous disease.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.