Evidence map›Paper›PMID 20085337›Full record

ArticleLangmuir : the ACS journal of surfaces and colloids2010

Imaging macromolecular interactions at an interface.

Joshua W Lampe, Zhengzheng Liao, Ivan J Dmochowski, Portonovo S Ayyaswamy, David M Eckmann

Abstract read
In one paragraph

Article in Langmuir : the ACS journal of surfaces and colloids, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
2.0field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 17 citations in OpenAlex.

  1. Article
  2. Visualizing monolayers with a water-soluble fluorophore to quantify adsorption, desorption, and the double layer.Proceedings of the National Academy of Sciences of the United States of America · 2015
    Article
  3. Article
  4. Article
  5. Article
  6. Protein assembly at the air-water interface studied by fluorescence microscopy.Langmuir : the ACS journal of surfaces and colloids · 2011
    Article
  7. Article
  8. Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Joshua W LampeCenter for Resuscitation Science, Department of Emergency Medicine, Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
Zhengzheng Liao
Ivan J Dmochowski
Portonovo S Ayyaswamy
David M Eckmann
University of Pennsylvania · USHospital of the University of Pennsylvania · US

Funding

INTERFACIAL MECHANICS IN INTRAVASCULAR GAS EMBOLISMR01HL060230 · NHLBI · UNIVERSITY OF PENNSYLVANIA · PI ECKMANN, DAVID M · 1999 to 2011
$3.8M
Oligonucleotides for turning genes on and off with lightR01GM083030 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI DMOCHOWSKI, IVAN JULIAN · 2008 to 2017
$3.0M
Activation of Clotting &cell adhesion : gas embolismR01HL067986 · NHLBI · UNIVERSITY OF PENNSYLVANIA · PI ECKMANN, DAVID M · 2002 to 2010
$2.4M
LEICA TCS SP2 AOBS UV CONFOCAL SYSTEM: EMBRYO DEVELOPMENTS10RR021113 · NCRR · UNIVERSITY OF PENNSYLVANIA · PI DMOCHOWSKI, IVAN JULIAN · 2005 to 2005
$497k
NCRR NIH HHS 1S10RR021113NCRR NIH HHS S10 RR021113NHLBI NIH HHS R01 HL060230NHLBI NIH HHS R01 HL067986NHLBI NIH HHS R01 HL60230NHLBI NIH HHS R01 HL67986NIGMS NIH HHS R01 GM083030
6 · The paper itself

Abstract

Important physiological, pathological, and technological processes occur at continuous and dispersed phase interfaces. Understanding these processes is limited by inability to quantitate molecular events occurring at the interface. To provide a model-independent measurement of protein concentration and mobility at the interface, we employed confocal laser scanning microscopy (CLSM). Fluorescently labeled albumin and fibrinogen were studied singly, pairwise, and with a surfactant, Pluronic F-127, in aqueous droplets. CLSM enables measurement of molecular behaviors manifest as surface inhomogeneity and of biophysical quantities including partitioning between the bulk and the gas-liquid (GL) interface. We conclude that albumin and fibrinogen behave substantially differently at the GL interface, adsorption from multispecies solutions is fundamentally different than adsorption from solutions of single species, and surfactants can inhibit proteins from occupying the interface.

Indexed as

AlbuminsFibrinogenMacromolecular SubstancesMicroscopy, ConfocalParticle SizePoloxamerSolutionsSurface PropertiesAlbuminsFibrinogenMacromolecular SubstancesPoloxamerSolutions

Identifiers

PMID20085337
PMCPMC2819646
OpenAlexW1980479545

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.