ArticlePlant cell reports2010
Isolation and functional characterization of two novel seed-specific promoters from sunflower (Helianthus annuus L.).
Article in Plant cell reports, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 48 citations in OpenAlex.
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Genome-Wide Tissue-Specific Genes Identification for Novel Tissue-Specific Promoters Discovery in Soybean.Genes · 2023Article
- Harnessing tissue-specific genome editing in plants through CRISPR/Cas system: current state and future prospects.Planta · 2021Review
- Identification of a 193 bp promoter region of TaNRX1-D gene from common wheat that contributes to osmotic or ABA stress inducibility in transgenic Arabidopsis.Genes & genomics · 2021Article
- Seed-specific activity of the Arabidopsis β-glucosidase 19 promoter in transgenic Arabidopsis and tobacco.Plant cell reports · 2021Article
- Cloning and Functional Characterization of a Pericarp Abundant Expression Promoter (Frontiers in genetics · 2021Article
- Identification of transcription factors interacting with a 1274 bp promoter of MaPIP1;1 which confers high-level gene expression and drought stress Inducibility in transgenic Arabidopsis thaliana.BMC plant biology · 2020Article
- An update and perspectives on the use of promoters in plant genetic engineering.Journal of biosciences · 2020Review
- Identification of Flower-Specific Promoters through Comparative Transcriptome Analysis inInternational journal of molecular sciences · 2019Article
- Identification of a 119-bp Promoter of the Maize Sulfite Oxidase Gene (International journal of molecular sciences · 2019Article
- Article
- Functional Characterization ofFrontiers in plant science · 2017Article
- Article
- Identification of a 467 bp Promoter of Maize Phosphatidylinositol Synthase Gene (ZmPIS) Which Confers High-Level Gene Expression and Salinity or Osmotic Stress Inducibility in Transgenic Tobacco.Frontiers in plant science · 2016Article
- Article
- Spatial and temporal activity of the foxtail millet (Setaria italica) seed-specific promoter pF128.Planta · 2015Article
- GmPRP2 promoter drives root-preferential expression in transgenic Arabidopsis and soybean hairy roots.BMC plant biology · 2014Article
- A Novel Moderate Constitutive Promoter Derived from Poplar (Populus tomentosa Carrière).International journal of molecular sciences · 2013Article
- The ABA signal transduction mechanism in commercial crops: learning from Arabidopsis.Plant cell reports · 2012Review
- Designer promoter: an artwork of cis engineering.Plant molecular biology · 2011Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The promoter region of two sunflower (Helianthus annuus L. HA89 genotype) seed specifically expressed genes, coding for an oleate desaturase (HaFAD2-1) and a lipid transfer protein (HaAP10), were cloned and in silico characterized. The isolated fragments are 867 and 964 bp long, respectively, and contain several seed-specific motifs, such as AACA motif, ACGT element, E-Boxes, SEF binding sites and GCN4 motif. Functional analysis of these promoters in transgenic Arabidopsis plants was investigated after fusing them with the beta-glucuronidase (GUS) reporter gene. None of the promoters triggered GUS activity in any vegetative tissue, with the exception of early seedling cotyledons. HaFAD2-1 and HaAP10 promoters were tested along seed development from globular stage to mature seeds. GUS staining was restricted to embryonic tissue and quantitative fluorometric assays showed high activity values at the later stages of development. In this work we demonstrate that HaFAD2-1 promoter is as strong as 35S promoter even though it is a tissue-specific promoter and its activity derived just from the embryo, thus confirming that it can be considered a strong highly specific seed promoter useful for biotechnology applications.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.