ArticleSensors and actuators. B, Chemical2009
A microwell array device capable of measuring single-cell oxygen consumption rates.
Article in Sensors and actuators. B, Chemical, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The trial behind it
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Who cites it
33 citing papers in PubMed.
- Size-related variability of oxygen consumption rates in individual human hepatic cells.Lab on a chip · 2024Article
- A Point-of-Care Nucleic Acid Quantification Method by Counting Light Spots Formed by LAMP Amplicons on a Paper Membrane.Biosensors · 2024Article
- Efficient single-cell oxygen consumption rate characterization based on frequency domain fluorescence lifetime imaging microscopy measurement and microfluidic platform.Biomicrofluidics · 2023Article
- Rapid fabrication of sieved microwells and cross-flow microparticle trapping.Scientific reports · 2020Article
- Optical Detection of Intracellular Quantities Using Nanoscale Technologies.Accounts of chemical research · 2019Article
- High-throughput, label-free, single-cell photoacoustic microscopy of intratumoral metabolic heterogeneity.Nature biomedical engineering · 2019Article
- Perfusion double-channel micropipette probes for oxygen flux mapping with single-cell resolution.Beilstein journal of nanotechnology · 2018Article
- Control of oxygen tension recapitulates zone-specific functions in human liver microphysiology systems.Experimental biology and medicine (Maywood, N.J.) · 2017Article
- A platform for high-throughput bioenergy production phenotype characterization in single cells.Scientific reports · 2017Article
- Microchambers with Solid-State Phosphorescent Sensor for Measuring Single Mitochondrial Respiration.Sensors (Basel, Switzerland) · 2016Article
- Modular Polymer Biosensors by Solvent Immersion Imprint Lithography.Journal of polymer science. Part B, Polymer physics · 2016Article
- Flexible Sheet-Type Sensor for Noninvasive Measurement of Cellular Oxygen Metabolism on a Culture Dish.PloS one · 2015Article
- Review of methods to probe single cell metabolism and bioenergetics.Metabolic engineering · 2015Review
- Dually fluorescent core-shell microgels for ratiometric imaging in live antigen-presenting cells.PloS one · 2014Article
- Emerging technologies for non-invasive quantification of physiological oxygen transport in plants.Planta · 2013Article
- Oxygen consumption rates of bacteria under nutrient-limited conditions.Applied and environmental microbiology · 2013Article
- Physical principles for scalable neural recording.Frontiers in computational neuroscience · 2013Article
- Micro-patterning and characterization of PHEMA-co-PAM-based optical chemical sensors for lab-on-a-chip applications.Sensors and actuators. B, Chemical · 2012Article
- High Throughput Micropatterning of Optical Oxygen Sensor for Single Cell Analysis.IEEE sensors journal · 2012Article
- Method for physiologic phenotype characterization at the single-cell level in non-interacting and interacting cells.Journal of biomedical optics · 2012Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Due to interest in cell population heterogeneity, the development of new technology and methodologies for studying single cells has dramatically increased in recent years. The ideal single cell measurement system would be high throughput for statistical relevance, would measure the most important cellular parameters, and minimize disruption of normal cell function. We have developed a microwell array device capable of measuring single cell oxygen consumption rates (OCR). This OCR device is able to diffusionally isolate single cells and enables the quantitative measurement of oxygen consumed by a single cell with fmol/min resolution in a non-invasive and relatively high throughput manner. A glass microwell array format containing fixed luminescent sensors allows for future incorporation of additional cellular parameter sensing capabilities. To demonstrate the utility of the OCR device, we determined the oxygen consumption rates of a small group of single cells (12 to 18) for three different cells lines: murine macrophage cell line RAW264.7, human epithelial lung cancer cell line A549, and human Barrett's esophagus cell line CP-D.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.