ArticleProtein engineering, design & selection : PEDS2010
Temperature dependence of the flexibility of thermophilic and mesophilic flavoenzymes of the nitroreductase fold.
Article in Protein engineering, design & selection : PEDS, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 39 citations in OpenAlex.
- Cloning, purification and possible use of a Bacillus nitroreductase in biotechnological applications.Scientific reports · 2026Article
- Catalytic His-loop flexibility drives high activity in hyperthermophilic esterase EstE1 while preserving structural stability.Microbiology spectrum · 2025Article
- Homologous Pairs of Low and High Temperature Originating Proteins Spanning the Known Prokaryotic Universe.Scientific data · 2023Article
- A Minireview on Temperature Dependent Protein Conformational Sampling.The protein journal · 2021Review
- Understanding thermal and organic solvent stability of thermoalkalophilic lipases: insights from computational predictions and experiments.Journal of molecular modeling · 2020Article
- Distinct roles of an ionic interaction holding an alpha-helix with catalytic Asp and a beta-strand with catalytic His in a hyperthermophilic esterase EstE1 and a mesophilic esterase rPPE.Extremophiles : life under extreme conditions · 2019Article
- Functional Characterization and Structural Analysis of NADH Oxidase Mutants fromMicroorganisms · 2019Article
- Insights from molecular dynamics simulations for computational protein design.Molecular systems design & engineering · 2017Article
- Conformational plasticity surrounding the active site of NADH oxidase from Thermus thermophilus.Protein science : a publication of the Protein Society · 2015Article
- Are coarse-grained models apt to detect protein thermal stability? The case of OPEP force field.Journal of non-crystalline solids · 2015Article
- How conformational flexibility stabilizes the hyperthermophilic elongation factor G-domain.The journal of physical chemistry. B · 2013Article
- Identification of a long-range protein network that modulates active site dynamics in extremophilic alcohol dehydrogenases.The Journal of biological chemistry · 2013Article
- Thermophilic proteins: insight and perspective from in silico experiments.Chemical Society reviews · 2012Review
- A temperature-dependent conformational change of NADH oxidase from Thermus thermophilus HB8.Proteins · 2012Article
- Using molecular dynamics to probe the structural basis for enhanced stability in thermal stable cytochromes P450.Biochemistry · 2010Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
A widely held hypothesis regarding the thermostability of thermophilic proteins states asserts that, at any given temperature, thermophilic proteins are more rigid than their mesophilic counterparts. Many experimental and computational studies have addressed this question with conflicting results. Here, we compare two homologous enzymes, one mesophilic (Escherichia coli FMN-dependent nitroreductase; NTR) and one thermophilic (Thermus thermophilus NADH oxidase; NOX), by multiple molecular dynamics simulations at temperatures from 5 to 100 degrees C. We find that the global rigidity/flexibility of the two proteins, assessed by a variety of metrics, is similar on the time scale of our simulations. However, the thermophilic enzyme retains its native conformation to a much greater degree at high temperature than does the mesophilic enzyme, both globally and within the active site. The simulations identify the helix F-helix G 'arm' as the region with the greatest difference in loss of native contacts between the two proteins with increasing temperature. In particular, a network of electrostatic interactions holds helix F to the body of the protein in the thermophilic protein, and this network is absent in the mesophilic counterpart.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.