ArticleInternational journal for parasitology2010
Members of the Entamoeba histolytica transmembrane kinase family play non-redundant roles in growth and phagocytosis.
Article in International journal for parasitology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 42 citations in OpenAlex.
- AGC family kinase of Entamoeba histolytica: Decoding the members biochemically.PLoS pathogens · 2024Article
- Live Cells Imaging and Comparative Phosphoproteomics Uncover Proteins from the Mechanobiome inInternational journal of molecular sciences · 2023Article
- A Review: Natural and Synthetic Compounds TargetingMembranes · 2022Review
- An atypical EhGEF regulates phagocytosis in Entamoeba histolytica through EhRho1.PLoS pathogens · 2021Article
- Review
- Article
- Entamoeba histolytica: Host parasite interactions at the colonic epithelium.Tissue barriers · 2017Article
- Imaging flow cytometry analysis of intracellular pathogens.Methods (San Diego, Calif.) · 2017Review
- Endoplasmic reticulum-resident Rab8A GTPase is involved in phagocytosis in the protozoan parasite Entamoeba histolytica.Cellular microbiology · 2016Article
- Knockdown of Five Genes Encoding Uncharacterized Proteins Inhibits Entamoeba histolytica Phagocytosis of Dead Host Cells.Infection and immunity · 2016Article
- Proteomic Study of Entamoeba histolytica Trophozoites, Cysts, and Cyst-Like Structures.PloS one · 2016Article
- Chew on this: amoebic trogocytosis and host cell killing by Entamoeba histolytica.Trends in parasitology · 2015Review
- Transferrin: Endocytosis and Cell Signaling in Parasitic Protozoa.BioMed research international · 2015Review
- A novel alpha kinase EhAK1 phosphorylates actin and regulates phagocytosis in Entamoeba histolytica.PLoS pathogens · 2014Article
- Crystal structure and putative substrate identification for the Entamoeba histolytica low molecular weight tyrosine phosphatase.Molecular and biochemical parasitology · 2014Article
- The cell surface proteome of Entamoeba histolytica.Molecular & cellular proteomics : MCP · 2014Article
- Article
- Transcriptome analysis of encystation in Entamoeba invadens.PloS one · 2013Article
- The Entamoeba histolytica serum-inducible transmembrane kinase EhTMKB1-9 is involved in intestinal amebiasis.International journal for parasitology. Drugs and drug resistance · 2012Article
- Control of Entamoeba histolytica adherence involves metallosurface protease 1, an M8 family surface metalloprotease with homology to leishmanolysin.Infection and immunity · 2012Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Entamoeba histolytica contains a large and novel family of transmembrane kinases (TMKs). The expression patterns of the E. histolytica TMKs in individual trophozoites and the roles of the TMKs for sensing and responding to extracellular cues were incompletely characterised. Here we provide evidence that single cells express multiple TMKs and that TMK39 and TMK54 likely serve non-redundant cellular functions. Laser-capture microdissection was used in conjunction with microarray analysis to demonstrate that single trophozoites express more than one TMK gene. Anti-peptide antibodies were raised against unique regions in the extracellular domains of TMK39, TMK54 and PaTMK, and TMK expression was analysed at the protein level. Flow cytometric assays revealed that populations of trophozoites homogeneously expressed TMK39, TMK54 and PaTMK, while confocal microscopy identified different patterns of cell surface expression for TMK39 and TMK54. The functions of TMK39 and TMK54 were probed by the inducible expression of dominant-negative mutants. While TMK39 co-localised with ingested beads and expression of truncated TMK39 interfered with trophozoite phagocytosis of apoptotic lymphocytes, expression of a truncated TMK54 inhibited growth of amoebae and altered the surface expression of the heavy subunit of the E. histolytica Gal/GalNAc lectin. Overall, our data indicates that multiple members of the novel E. histolytica TMK family are utilised for non-redundant functions by the parasite.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.